Veterinarski arhiv, Vol. 91 No. 2, 2021.
Izvorni znanstveni članak
https://doi.org/10.24099/vet.arhiv.0914
Determination of thiol/disulphide homeostasis as a new indicator of oxidative stress in dairy cows with subclinical endometritis
Birten Emre
; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Harran University, Sanliurfa, Turkey
Ömer Korkmaz
; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Harran University, Sanliurfa, Turkey
Ismail Koyuncu
; Department of Biochemistry, Faculty of Medicine, Harran University, Sanliurfa, Turkey
Selim Çomaklı
; Department of Biometry, Faculty of Veterinary Medicine, Erciyes University, Kayseri, Turkey
Aytaç Akçay
; Department of Pathology, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey
Abuzer K. Zonturlu
; Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Harran University, Sanliurfa, Turkey
Özcan Erel
; Department of Biochemistry, Faculty of Medicine, Yıldırım Beyazıt University, Ankara, Turkey
Sažetak
The objective of this study was to determine thiol/disulphide homeostasis (TDH) in infertile cows with subclinical endometritis (SCE). Endometrial cytological samples were collected using a cytobrush to diagnose SCE in 36 infertile cows. According to the results of the cytology examination, those with acute endometritis were classified as Group I (n = 20) and those with chronic endometritis were classified as Group II (n = 16). A control group was formed of heifers as Group III (n = 20). Blood samples were taken from each group on the day of diagnosis (day 0) to analyse TDH. In the cytology examination, both the Giemsa method and immunocytochemical staining were applied to determine chronic inflammation and activity status. In 55.55% (20/36) of the infertile cows with cytological endometritis, the inflammation was determined to be active, and in 44.44% (16/36) it had become chronic. The native thiol and total thiol levels were found to be statistically significantly lower in the acute (206.54 ± 8.30 μmol/L; 227.11 ± 9.30 μmol/L) and chronic SCE cases (225.15 ± 11.89 μmol/L; 247.96 ± 10.80 μmol/L) compared to the heathy control group (308.47 ±
13.59 μmol/L; 336.83 ± 15.5 μmol/L respectively) (P<0.001). Disulphide levels, disulphide/total thiol, native thiol/ total thiol and disulphide/native thiol ratios were similar in all the groups (P>0.05). The diagnostic accuracy of native thiol, which can be used in the diagnosis of SCE, was 92.8%, that of total thiol was 89.3% and that of disulphide was 64.3% according to the ROC curve analysis. These results demonstrate that TDH is a reliable and sensitive indicator of oxidative stress in cow SCE, and that abnormal TDH might play a role in SCE pathogenic mechanisms. This is the first study to evaluate thiol/disulphide homeostasis in dairy cows with SCE as a new indicator of oxidative stress.
Ključne riječi
cow; oxidative stress; ROC; subclinical endometritis; thiol/disulphide
Hrčak ID:
258459
URI
Datum izdavanja:
8.6.2021.
Posjeta: 656 *