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https://doi.org/10.37427/botcro-2021-024

Development of an optimum proliferation medium via the graph kernel statistical analysis method for genetically stable in vitro propagation of endemic Thymus cilicicus (Turkey)

Ergun Kaya   ORCID icon orcid.org/0000-0003-4255-3802 ; Mugla Sitki Kocman University, Faculty of Science, 48000, Kotekli, Mugla, Turkey
Mehmet Ali Balci ; Mugla Sitki Kocman University, Faculty of Science, 48000, Kotekli, Mugla, Turkey
Omer Akguller   ORCID icon orcid.org/0000-0002-7061-2534 ; Mugla Sitki Kocman University, Faculty of Science, 48000, Kotekli, Mugla, Turkey
Selin Galatali ; Mugla Sitki Kocman University, Faculty of Science, 48000, Kotekli, Mugla, Turkey
Sevil Yeniocak ; Mugla Sitki Kocman University, Faculty of Science, 48000, Kotekli, Mugla, Turkey
Taner Mercan   ORCID icon orcid.org/0000-0003-1187-8284 ; Mugla Sitki Kocman University, Faculty of Science, 48000, Kotekli, Mugla, Turkey
Sevinc Guldag   ORCID icon orcid.org/0000-0001-8808-172X ; Mugla Sitki Kocman University, Faculty of Science, 48000, Kotekli, Mugla, Turkey
Damla Ekin Ozkaya   ORCID icon orcid.org/0000-0002-2952-1999 ; Mugla Sitki Kocman University, Faculty of Science, 48000, Kotekli, Mugla, Turkey
Bilge Ozturk   ORCID icon orcid.org/0000-0002-0403-1037 ; Mugla Sitki Kocman University, Faculty of Science, 48000, Kotekli, Mugla, Turkey
Onur Celik   ORCID icon orcid.org/0000-0002-1040-9833 ; Mugla Sitki Kocman University, Faculty of Science, 48000, Kotekli, Mugla, Turkey
Irem Aktay   ORCID icon orcid.org/0000-0002-7615-0123 ; Mugla Sitki Kocman University, Faculty of Science, 48000, Kotekli, Mugla, Turkey

Puni tekst: engleski, pdf (3 MB) str. 199-207 preuzimanja: 24* citiraj
APA 6th Edition
Kaya, E., Ali Balci, M., Akguller, O., Galatali, S., Yeniocak, S., Mercan, T., ... Aktay, I. (2021). Development of an optimum proliferation medium via the graph kernel statistical analysis method for genetically stable in vitro propagation of endemic Thymus cilicicus (Turkey). Acta Botanica Croatica, 80 (2), 199-207. https://doi.org/10.37427/botcro-2021-024
MLA 8th Edition
Kaya, Ergun, et al. "Development of an optimum proliferation medium via the graph kernel statistical analysis method for genetically stable in vitro propagation of endemic Thymus cilicicus (Turkey)." Acta Botanica Croatica, vol. 80, br. 2, 2021, str. 199-207. https://doi.org/10.37427/botcro-2021-024. Citirano 17.10.2021.
Chicago 17th Edition
Kaya, Ergun, Mehmet Ali Balci, Omer Akguller, Selin Galatali, Sevil Yeniocak, Taner Mercan, Sevinc Guldag, et al. "Development of an optimum proliferation medium via the graph kernel statistical analysis method for genetically stable in vitro propagation of endemic Thymus cilicicus (Turkey)." Acta Botanica Croatica 80, br. 2 (2021): 199-207. https://doi.org/10.37427/botcro-2021-024
Harvard
Kaya, E., et al. (2021). 'Development of an optimum proliferation medium via the graph kernel statistical analysis method for genetically stable in vitro propagation of endemic Thymus cilicicus (Turkey)', Acta Botanica Croatica, 80(2), str. 199-207. https://doi.org/10.37427/botcro-2021-024
Vancouver
Kaya E, Ali Balci M, Akguller O, Galatali S, Yeniocak S, Mercan T i sur. Development of an optimum proliferation medium via the graph kernel statistical analysis method for genetically stable in vitro propagation of endemic Thymus cilicicus (Turkey). Acta Botanica Croatica [Internet]. 2021 [pristupljeno 17.10.2021.];80(2):199-207. https://doi.org/10.37427/botcro-2021-024
IEEE
E. Kaya, et al., "Development of an optimum proliferation medium via the graph kernel statistical analysis method for genetically stable in vitro propagation of endemic Thymus cilicicus (Turkey)", Acta Botanica Croatica, vol.80, br. 2, str. 199-207, 2021. [Online]. https://doi.org/10.37427/botcro-2021-024

Sažetak
Thymus cilicicus is an endemic Eastern Mediterranean element that has aromatic-medicinal properties. Its natural population spreads across gravelly ground and open rocky areas of South and Southwest Anatolia. The current study on in vitro propagation of T. cilicicus focused deeply on environmental applications such as the development of an optimum medium composition for efficient and genetically stable micropropagation and improved preservation procedures for long-time conservation of elite germplasms for further studies. For this purpose, MS and OM media were used individually and in combination with cytokinins, charcoal, AgNO3, Fe-EDDHA, and H3BO3. The raw data were statistically analyzed via the graph kernel method to optimize the nonlinear relationship between all parameters. The optimal proliferation medium for T. cilicicus was OM supplemented with a combination of 10 g L-1 charcoal and 1 mg L-1 KIN and the calculated averages of the best regeneration rate, the best shoot number and the best shoot length were 96.89%, 3 and 1.24 respectively on this medium. The determination of genetic stability of in vitro grown plants on the optimum medium compositions obtained by the graph kernel method was carried out with the use of the ISSR-PCR technique. All the ISSR primers produced a total of 192 reproductive band profiles, none of which were polymorphic. Furthermore, the micropropagated plants were successfully rooted and acclimatized to greenhouse conditions. In this study, we present a graph kernel multiple propagation index which considers all the possible parameters needing to be analyzed. Such an index is used for the first time for the determination of the optimum proliferation medium.

Ključne riječi
AgNO3; charcoal; Fe-EDDHA; graph kernel; H3BO3; micropropagation; thyme

Hrčak ID: 262325

URI
https://hrcak.srce.hr/262325

Posjeta: 60 *