Pregledni rad
Determination of total bacterial count in raw milk by flow cytometry
Dubravka Samaržija
; Zavod za mljekarstvo, Agronomski fakultet, Svetošimunska 25, 10000 Zagreb
Neven Antunac
Tomislav Pogačić
Sanja Sikora
Sažetak
The automatic flow cytometry as routine method for total bacterial count determination of raw ex-farm milk has recently been accepted in Croatia. This method significantly differs from the reference method (Standard Plate Count) mostly in the presentation of the results obtained. Therefore, this paper summarized experiences in the application of flow cytometry in the dairy laboratories practice. The principle and the practice of the method, methodological details and factors influencing the results were described. In order to avoid problems regarding the interpretation of the results, which are
general problems of the quantitative microbiology, this article try to explain an appropriate conversion of the results with regards to SPC/ml, as an official method for the bacteriological quality proposal by the national legislation.
Ključne riječi
total bacterial count; flow cytometry; accuracy of the method; reference method
Hrčak ID:
1509
URI
Datum izdavanja:
12.1.2004.
Posjeta: 7.023 *