Izvorni znanstveni članak
Nicotinamide Adenine Dinucleotide Phosphate-Diaphorase (NADPH-d) Histochemistry Detecting NOS in Healthy and Chronically Inflamed Pulp
S. Jukić
J. Talan-Hranilović
D. Buković
I. Miletić
E. Neziri
Sažetak
The aim of this study was to examine the expression of nicotinamide adenine dinucleotide
phosphate-diaphorase (NADPH-d) activity in human dental pulps and determine
whether there are changes of the activity in chronically inflamed pulp tissue. Nineteen
pulps with clinical diagnosis of chronic pulpitis were collected during endodontic
treatment. The healthy controls were obtained from teeth extracted for orthodontic
therapy. The clinical diagnosis was confirmed by histological analysis. Healthy pulps
showed stratified odontoblasts in peripheral parts, while in central area there was normal
connective tissue. Chronically inflamed pulps showed less expressed stratification
of odontoblasts and infiltration of lymphocytes, polymorphonuclear leukocytes, plasma
cells and mastocytes. NADPH-d granular reactivity was assessed semi quantitatively
under the light microscope by a single observer and scored on an intensity scale from
negative reaction to very strong reaction. In healthy human pulps, NADPH-d activity
was strong to very strong in odontoblastic layer. Endothelial cells and Schwann cells
showed strong NADPH-d reactivity, while the other parts of central area were weakly
positive. Similar distribution of reactivity was expressed also in chronically inflamed
pulp; moderate to strong reaction was observed in stromal area as result of positive reaction
in inflammatory cells and endothelial cells of abundant newly formed capillaries.
Ključne riječi
Hrčak ID:
28316
URI
Datum izdavanja:
16.12.2002.
Posjeta: 1.019 *