Izvorni znanstveni članak

Correlation between serum butyrylcholinesterase activity and serum lipid concentrations in rats treated with different antagonists of the adrenergic system

Žarka Krnić ; Istraživački institut PLIVA, Zagreb, Hrvatska
Mirjana Kujundžić-Tiljak ; Škola narodnog zdravlja
Renata Zrinski Topić ; Dječja bolnica „Srebrnjak“, Zagreb, Referentni centar Ministarstva zdravstva za kliničku alergologiju djece, Zagreb, Hrvatska
Vlasta Bradamante ; Medicinski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska

Sažetak

Background and Purpose: Based on the facts that the blockade of adrenergic receptors can alter lipid profile in the serum and that it has been suggested that butyrylcholinesterase (BuChE) is involved in lipid metabolism, different adrenergic blocking agents were administered to rats to modify lipid concentrations in serum. The activity of BuChE was examined under such conditions and correlations with serum lipids were investigated. The purpose of this study was to evaluate the effects of different adrenergic antagonists on BuChE activity and to investigate the correlation between BuChE activity and serum lipids.

Materials and Methods: Six groups of male Fischer 344 rats (9 animals/ group) were treated orally with adrenergic antagonists (mixed in commercial diet) during 6 weeks: oxprenolol, atenolol, doxazosin, oxprenolol and doxazosin, atenolol and doxazosin, and guanethidine. A control group (9 rats) received only commercial diet. BuChE activity in serum was determined with kinetic color test using butyrylthiocholine as a substrate. Concentrations of serum lipids (total cholesterol, triglycerides and HDL cholesterol) were determined by enzymatic colorimetric tests. Data were analyzed by Kruskal-Wallis test and Spearman’s correlation coefficient.

Results: The results revealed that oxprenolol and doxazosin (given alone or in combination with atenolol or oxprenolol) increased (>30 %) BuChE activity. BuChE activity correlated with different serum lipids, and correlation depended on the type of adrenergic blockade.

Conclusion: Although the examined adrenergic antagonists did not influence serum lipid concentrations, the increase of BuChE activity and correlation with serum lipid concentrations suggested that the increase of this enzyme’s activity might be the first sign of altered lipid metabolism.

Ključne riječi

Hrčak ID:

29077

URI

https://hrcak.srce.hr/29077

Datum izdavanja:

29.2.2008.

Posjeta: 1.525 *