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Measurement of malondialdehyde (MDA) level in rat plasma after simvastatin treatment using two different analytical methods

Jasna Lovrić orcid.org/0000-0002-3508-0915 ; Medicinski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Milan Mesić ; Istraživački centar GSK Zagreb LTD, Zagreb, Hrvatska
Marija Macan ; Medicinski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Marijan Koprivanac ; Medicinski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Marta Kelava ; Student medicine Sveučilišta u Zagrebu, Zagreb, Hrvatska
Vlasta Bradamante ; Medicinski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska

Sažetak

Background and Purpose: The aim of this study was to investigate the effect of chronic administration of simvastatin (SIMV) on plasma malondialdehyde (MDA) level using two different methods. We also wanted to examine the plasma MDA level 10 days after the last administration of SIMV.

Materials and Methods: The first two groups ofWistar rats were given 10 mg/kg/day of SIMV and the third and fourth groups 50 mg/kg/day of SIMV for 21 days. Two control groups were on saline for the same period. Plasma MDA level was measured after the end of treatment and 10 days after the last dose. Two methods were used: UV-VIS spectrophotometric method and HPLC-MS method. Statistics: Kruskal-Wallis test and Steel test for post-hoc comparison with the control group. P values less or equal to 0.05 were considered as statistically significant.

Results:MDA levels in all groups,measured by both techniques, showed that SIMV treatment caused a dose-dependent decrease (significant in high dose) in plasma MDA level. The decrease in MDA level was also wellmaintained for 10 days after the last administration of SIMV in both doses (significantly in high dose).

Conclusion: Both doses of SIMV decreased plasma MDA level after 21 day treatment and it remained decreased 10 days after the last dose, regardless of the measurement method used. These results showed that SIMV has antioxidant activity that persists after discontinuation of therapy

Ključne riječi

statins; simvastatin; malondialdehyde; lipid peroxidation; HPLC-MS; spectrophotometry; oxidative stress

Hrčak ID:

29090

URI

https://hrcak.srce.hr/29090

Datum izdavanja:

29.2.2008.

Posjeta: 18.897 *