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Arhiv za higijenu rada i toksikologiju, Vol.59 No.4 Prosinac 2008.

Kratko priopćenje
https://doi.org/10.2478/10004-1254-59-2008-1879

Determination of 8-xydroxy-2’deoxyguanosine in Urine Using HPLC With Electrochemical Detection

Ana-Marija Domijan ; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Maja Peraica ; Institute for Medical Research and Occupational Health, Zagreb, Croatia

Puni tekst: engleski, pdf (99 KB) str. 277-282 preuzimanja: 1.489* citiraj
APA 6th Edition
Domijan, A. i Peraica, M. (2008). Determination of 8-xydroxy-2’deoxyguanosine in Urine Using HPLC With Electrochemical Detection. Arhiv za higijenu rada i toksikologiju, 59 (4), 277-282. https://doi.org/10.2478/10004-1254-59-2008-1879
MLA 8th Edition
Domijan, Ana-Marija i Maja Peraica. "Determination of 8-xydroxy-2’deoxyguanosine in Urine Using HPLC With Electrochemical Detection." Arhiv za higijenu rada i toksikologiju, vol. 59, br. 4, 2008, str. 277-282. https://doi.org/10.2478/10004-1254-59-2008-1879. Citirano 13.12.2018.
Chicago 17th Edition
Domijan, Ana-Marija i Maja Peraica. "Determination of 8-xydroxy-2’deoxyguanosine in Urine Using HPLC With Electrochemical Detection." Arhiv za higijenu rada i toksikologiju 59, br. 4 (2008): 277-282. https://doi.org/10.2478/10004-1254-59-2008-1879
Harvard
Domijan, A., i Peraica, M. (2008). 'Determination of 8-xydroxy-2’deoxyguanosine in Urine Using HPLC With Electrochemical Detection', Arhiv za higijenu rada i toksikologiju, 59(4), str. 277-282. doi: https://doi.org/10.2478/10004-1254-59-2008-1879
Vancouver
Domijan A, Peraica M. Determination of 8-xydroxy-2’deoxyguanosine in Urine Using HPLC With Electrochemical Detection. Arh Hig Rada Toksikol. [Internet]. 2008 [pristupljeno 13.12.2018.];59(4):277-282. doi: https://doi.org/10.2478/10004-1254-59-2008-1879
IEEE
A. Domijan i M. Peraica, "Determination of 8-xydroxy-2’deoxyguanosine in Urine Using HPLC With Electrochemical Detection", Arhiv za higijenu rada i toksikologiju, vol.59, br. 4, str. 277-282, 2008. [Online]. doi: https://doi.org/10.2478/10004-1254-59-2008-1879

Sažetak
Endogenous cell metabolism and different chemicals, drugs, ionising radiation, solar light, cigarette smoking, and air pollution can induce oxidative damage to DNA. Oxidative DNA damage is involved in the development of different diseases, aging, and cancer. Urine 8-hydroxy-2’deoxyguanosine (8-oxodG) is widely accepted as a biomarker of the “whole body” oxidative DNA damage. The aim of this study was to introduce a method for determination 8-oxodG in spot urine samples using high-performance liquid chromatography with electrochemical detection. In the tested range of 50 nmol L-1 to 400 nmol L-1, the calibration curve was linear (r2=0.9995) and the limit of detection was 3 nmol L-1. Concentrations of 8-oxodG ranged between 3.0 nmol L-1 and 48.0 nmol L-1, which is in accordance with values reported for healthy individuals in other studies.

Ključne riječi
biomarker; free radicals; oxidative DNA damage; 8-oxodG

Hrčak ID: 29560

URI
https://hrcak.srce.hr/29560

[hrvatski]

Posjeta: 1.774 *