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A New Method for Detection of HNE-histidine Conjugates in Rat Inflammatory Cells

Morana Živković
Kamelija Žarković
Ljubomir Škrinjar
Georg Waeg
Marija Poljak-Blaži
Suzana Borović Šunjić
Rudolf Jörg Schaur
Neven Žarković

Puni tekst: engleski, pdf (399 KB) str. 91-98 preuzimanja: 411* citiraj
APA 6th Edition
Živković, M., Žarković, K., Škrinjar, Lj., Waeg, G., Poljak-Blaži, M., Borović Šunjić, S., ... Žarković, N. (2005). A New Method for Detection of HNE-histidine Conjugates in Rat Inflammatory Cells. Croatica Chemica Acta, 78 (1), 91-98. Preuzeto s https://hrcak.srce.hr/2796
MLA 8th Edition
Živković, Morana, et al. "A New Method for Detection of HNE-histidine Conjugates in Rat Inflammatory Cells." Croatica Chemica Acta, vol. 78, br. 1, 2005, str. 91-98. https://hrcak.srce.hr/2796. Citirano 08.03.2021.
Chicago 17th Edition
Živković, Morana, Kamelija Žarković, Ljubomir Škrinjar, Georg Waeg, Marija Poljak-Blaži, Suzana Borović Šunjić, Rudolf Jörg Schaur i Neven Žarković. "A New Method for Detection of HNE-histidine Conjugates in Rat Inflammatory Cells." Croatica Chemica Acta 78, br. 1 (2005): 91-98. https://hrcak.srce.hr/2796
Harvard
Živković, M., et al. (2005). 'A New Method for Detection of HNE-histidine Conjugates in Rat Inflammatory Cells', Croatica Chemica Acta, 78(1), str. 91-98. Preuzeto s: https://hrcak.srce.hr/2796 (Datum pristupa: 08.03.2021.)
Vancouver
Živković M, Žarković K, Škrinjar Lj, Waeg G, Poljak-Blaži M, Borović Šunjić S i sur. A New Method for Detection of HNE-histidine Conjugates in Rat Inflammatory Cells. Croatica Chemica Acta [Internet]. 2005 [pristupljeno 08.03.2021.];78(1):91-98. Dostupno na: https://hrcak.srce.hr/2796
IEEE
M. Živković, et al., "A New Method for Detection of HNE-histidine Conjugates in Rat Inflammatory Cells", Croatica Chemica Acta, vol.78, br. 1, str. 91-98, 2005. [Online]. Dostupno na: https://hrcak.srce.hr/2796. [Citirano: 08.03.2021.]

Sažetak
Oxidative stress, excessive production of reactive oxygen species, is considered an important part of different disorders, as well as of physiological processes (inflammation). The difference between physiological and pathological oxidative stress is often the occurrence of lipid peroxidation and its final toxic products, among which is 4-hydroxy-2-nonenal (HNE), a reactive aldehyde that forms protein conjugates. The aim of this study was to determine the distribution of HNE-histidine conjugates in leukocytes during systemic inflammation. We used genuine monoclonal antibodies against HNE-histidine conjugates for immuno-cytochemical, immuno- histochemical and immuno-electronmicroscopical analyses of HNE in inflammatory cells. Spleen tissue, leukocytes from blood and macrophages from the peritoneum of rats intraperitoneally (i.p.) injected with micronized zeolite (MZ) were analyzed. HNE-histidine conjugates were predominantly detected near cell membranes, phagosomes and macrophage granules. Immunodetection of HNE-histidine conjugates may be used as an analytical immunochemical method to study HNE formation in pathological and physiological processes and for pathomorphological diagnostic procedures.

Ključne riječi
4-hydroxy-2-nonenal; oxidative stress; leukocytes; immunogold method

Hrčak ID: 2796

URI
https://hrcak.srce.hr/2796

[hrvatski]

Posjeta: 909 *