Izvorni znanstveni članak
INFLUENCE OF FILTRATION OR CENTRIFUGATION OF SEMEN DILUENT ON THE VIABILITY OF CHILLED BUFFALO-BULL SEMEN
H. Amer
; Department of Technology, Faculty of Veterinary Medicine, Zagazig University.
H. Mansour
; Department of Technology, Faculty of Veterinary Medicine, Zagazig University
A. El-Sayed
; Department of Technology, Faculty of Veterinary Medicine, Zagazig University
A. Swelum
; Department of Technology, Faculty of Veterinary Medicine, Zagazig University
Sažetak
This study was conducted to investigate influence of filtration or centrifugation of TRIS milk diluent on the viability and acrosomal integrity of buffalo spermatozoa preserved under refrigeration condition. Semen ejaculates (n=40) were collected twice weekly using artificial vagina. Each ejaculate was exposed to the following diluents, TRIS (T1 to T4), Milk (M1 to M8), and combinations of TRIS and milk diluents at different ratios (TM1 to TM11). Some parameters as live percentage, viability indicies and acrosomal integrity of spermatozoa was calculated immediately following dilution of semen, then at 3rd, 6th and 9th day of storage at refrigeration temperature (5ºC). Data were statistically analyzed. The results revealed that centrifugation of TRIS diluent once (T3) or twice (T4), before dilution processes, had a beneficial effect on the examined parameters of diluted semen (live percentage, viability indicies and acrosomal integrity of spermatozoa) during the entire period of storage when compared with control TRIS diluent (T1) or filtrated TRIS diluent (T2). However, T3 was the best of all (T1, T2 or T4). Centrifugation of milk-yolk diluent (M3) was found better than any other treatment for the examined milk diluents. Moreover, M3 was the best of all followed by M4 and M6, then followed by the others. Likewise, a combination of T3 and M3 at a ratio of 50:50 (TM6) was, in many aspects, superior to any other combination (TM1 to TM11) or diluents used in this study for dilution and preservation of buffalo semen at refrigeration temperature. It can be concluded that centrifugation of TRIS or milk diluent once before dilution would be the best treatment. Moreover, combination of one part of centrifuged TRIS diluent and one part of centrifuged milk diluents proved to be the proper diluent of choice for dilution and preservation of buffalo semen. It is recommended to use this combination to dilute and freeze buffalo semen for the artificial insemination program.
Ključne riječi
Buffalo bull; semen; filtration; centrifugation; preservation; fertilizing; capacity
Hrčak ID:
33014
URI
Datum izdavanja:
15.12.2008.
Posjeta: 1.522 *