Croatica Chemica Acta, Vol. 78 No. 3, 2005.
Izvorni znanstveni članak
Effects of Aspirin and Indomethacin on Galectin-3
Sanja Dabelić
Mirna Flögel
Jerka Dumić
Sažetak
Galectin-3, a β-galactoside binding lectin, acts as a strong pro inflammatory signal. Many immunomodulatory drugs affect signaling pathways that comprise transcription factors involved in regulation of galectin-3 gene (LGALS3) expression. The aim of this study was to investigate the effects of non-steroidal anti-inflammatory drugs aspirin and indomethacin on the expression of galectin-3, both on mRNA and protein levels. The human monocytic cell line THP-1 was exposed to various therapeutic concentrations of aspirin and indomethacin for 72 hours. Relative RT-PCR method and the GeneScan analysis software were used for assessing the galectin-3 mRNA level and chemiluminescent-western blot analysis was used for measuring the galectin-3 level. The results showed that galectin-3 is a new target molecule of NSAIDs, which cause reduction of both gene and protein expression of galectin-3, but the intensity and time-course of the changes strongly depend on the kind and concentration of the drugs.
Ključne riječi
aspirin; galectin-3; indomethacin; LGALS3
Hrčak ID:
54
URI
Datum izdavanja:
15.9.2005.
Posjeta: 1.935 *