Skoči na glavni sadržaj

Izvorni znanstveni članak

https://doi.org/10.2478/10004-1254-60-2009-1891

Disturbance of Cell Proliferation in Response to Mobile Phone Frequency Radiation

Ivančica Trošić ; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Ivan Pavičić ; Institute for Medical Research and Occupational Health, Zagreb, Croatia


Puni tekst: engleski pdf 166 Kb

str. 109-115

preuzimanja: 949

citiraj


Sažetak

The aim of study was to determine the infl uence of mobile phone frequency radiation on the proliferation, cytoskeleton structure, and mitotic index of V79 cells after 1 h, 2 h, and 3 h of exposure. V79 cells were cultured in standard laboratory conditions and exposed to continuous-wave (CW) RF/MW radiation of 935 MHz, electric fi eld strength of (8.2±0.3) V m-1, and specifi c absorption rate (SAR) of 0.12 W kg-1. To identify proliferation kinetics, the cells were counted for each hour of exposure 24 h, 48 h, 72 h, and 96 h after respective exposures. Microtubule proteins were determined using specifi c immunocytochemical methods. Cell smears were analysed under a fl uorescent microscope. The study included negative and positive controls. Mitotic index was determined by estimating the number of dividing cells 24 h after exposure and dividing it with the total number of cells. In comparison to the controls, cell proliferation declined in cells exposed for three hours 72 h after irradiation (p<0.05). Microtubule structure was clearly altered immediately after three hours of irradiation (p<0.05). The mitotic index in RF/MW-exposed cells did not differ from negative controls. However, even if exposure did not affect the number of dividing cells, it may have slowed down cell division kinetics as a consequence of microtubule impairment immediately after exposure.

Ključne riječi

continuous cell culture; microtubule structure; mitotic index; RF/MW; 935 MHz

Hrčak ID:

34876

URI

https://hrcak.srce.hr/34876

Datum izdavanja:

27.3.2009.

Podaci na drugim jezicima: hrvatski

Posjeta: 2.083 *