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Long-Term Bone Marrow Culture

Silvana Stanović
Milivoj Boranić

Puni tekst: hrvatski, pdf (292 KB) str. 265-275 preuzimanja: 924* citiraj
APA 6th Edition
Stanović, S. i Boranić, M. (1998). Dugotrajna kultura koštane srži. Arhiv za higijenu rada i toksikologiju, 49 (3), 265-275. Preuzeto s https://hrcak.srce.hr/3292
MLA 8th Edition
Stanović, Silvana i Milivoj Boranić. "Dugotrajna kultura koštane srži." Arhiv za higijenu rada i toksikologiju, vol. 49, br. 3, 1998, str. 265-275. https://hrcak.srce.hr/3292. Citirano 16.06.2019.
Chicago 17th Edition
Stanović, Silvana i Milivoj Boranić. "Dugotrajna kultura koštane srži." Arhiv za higijenu rada i toksikologiju 49, br. 3 (1998): 265-275. https://hrcak.srce.hr/3292
Harvard
Stanović, S., i Boranić, M. (1998). 'Dugotrajna kultura koštane srži', Arhiv za higijenu rada i toksikologiju, 49(3), str. 265-275. Preuzeto s: https://hrcak.srce.hr/3292 (Datum pristupa: 16.06.2019.)
Vancouver
Stanović S, Boranić M. Dugotrajna kultura koštane srži. Arh Hig Rada Toksikol. [Internet]. 1998 [pristupljeno 16.06.2019.];49(3):265-275. Dostupno na: https://hrcak.srce.hr/3292
IEEE
S. Stanović i M. Boranić, "Dugotrajna kultura koštane srži", Arhiv za higijenu rada i toksikologiju, vol.49, br. 3, str. 265-275, 1998. [Online]. Dostupno na: https://hrcak.srce.hr/3292. [Citirano: 16.06.2019.]

Sažetak
Long-term bone marrow culture is an experimental in vitro model of hematopoiesis imitating conditions in vivo. It contains hematopoietic elements at various stages of differentiation as well as a supportive stromal microenvironment. Primitive hematopoietic stem cells of mesenchymal origin, the long-term culture initiating cells proliferate and differentiate into different cell types, giving rise to the adherent stromal layer and to various hematopoietic elements attached to it or floating freely in the supernatant medium. The stromal layer keeps the hematopoietic cells aggregated, helps their mitosis, differentiation and maturation by cell-to-cell contact, produces hematopoietic growth factors (cytokines), and forms the extracellular matrix required for cell attachment. Hematopoiesis occurs without exogenous growth factors. The appearance and development of the stroma, the proliferation and differentiation of hematopoietic progenitor cells, and the production of cytokines differ in long-term cultures of the normal and of pathologically altered bone marrow. Long-term bone marrow culture is applied in fundamental studies of normal and pathologically altered hematopoiesis, in pharmacological research, in the purging of residual leukemia cells from bone marrow autotransplants, and in the gene transfer. It is also suitable for testing carcinogenic and toxic chemicals causing hematopoietic damage through occupational or habitual exposure.

Ključne riječi
antineoplastic agents; bone marrow; cell; cell culture; cell proliferation and differentiation; hematopoiesis; hematopoietic toxicity

Hrčak ID: 3292

URI
https://hrcak.srce.hr/3292

[hrvatski]

Posjeta: 1.353 *