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Standardisation of instrumentation in plant DNA image cytometry

B. Vilhar
M. Dermastia

Puni tekst: engleski, pdf (460 KB) str. 11-26 preuzimanja: 961* citiraj
APA 6th Edition
Vilhar, B. i Dermastia, M. (2002). Standardisation of instrumentation in plant DNA image cytometry. Acta Botanica Croatica, 61 (1), 11-26. Preuzeto s https://hrcak.srce.hr/3484
MLA 8th Edition
Vilhar, B. i M. Dermastia. "Standardisation of instrumentation in plant DNA image cytometry." Acta Botanica Croatica, vol. 61, br. 1, 2002, str. 11-26. https://hrcak.srce.hr/3484. Citirano 19.10.2021.
Chicago 17th Edition
Vilhar, B. i M. Dermastia. "Standardisation of instrumentation in plant DNA image cytometry." Acta Botanica Croatica 61, br. 1 (2002): 11-26. https://hrcak.srce.hr/3484
Harvard
Vilhar, B., i Dermastia, M. (2002). 'Standardisation of instrumentation in plant DNA image cytometry', Acta Botanica Croatica, 61(1), str. 11-26. Preuzeto s: https://hrcak.srce.hr/3484 (Datum pristupa: 19.10.2021.)
Vancouver
Vilhar B, Dermastia M. Standardisation of instrumentation in plant DNA image cytometry. Acta Botanica Croatica [Internet]. 2002 [pristupljeno 19.10.2021.];61(1):11-26. Dostupno na: https://hrcak.srce.hr/3484
IEEE
B. Vilhar i M. Dermastia, "Standardisation of instrumentation in plant DNA image cytometry", Acta Botanica Croatica, vol.61, br. 1, str. 11-26, 2002. [Online]. Dostupno na: https://hrcak.srce.hr/3484. [Citirano: 19.10.2021.]

Sažetak
DNAimage cytometry is a relatively new technique for densitometric measurement of nuclear DNAcontent, which has only rarely been used in botany and thus no methodological standards exist for this method to be applied to the measurement of plant material. In the
present paper we address several problems related to standardisation of DNA image cytometry, such as stability of the measuring system, linearity of optical density measurements,
correction of uneven illumination of the field of view, and uniformity of integrated optical density measurement over the entire field of view. Furthermore, image processing procedures are described for mitigation of the effects of electronic noise (image averaging)
and for densitometric calibration of the measuring system. We have developed a macro for plant DNAimage cytometry, using a general image analysis software package. The described quality control procedures, adopted from international medical standards
for diagnostic DNAimage cytometry, were used during software development to test performance of our measurement system. Until a specific botanical consensus is reached, we recommend that the quality control standards for instrumentation described in the present
paper are considered when DNA image cytometry is used for measurement of plant genome size as well as for any other image analysis-related densitometric measurement based on light microscopy.

Ključne riječi
DNA image cytometry; standardisation; instrumentation; nuclear DNA content; plant material

Hrčak ID: 3484

URI
https://hrcak.srce.hr/3484

Posjeta: 1.313 *