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p - 16 INK4a as a marker for cervical dysplasia

Ana Krvavica ; General Hospital Zadar, Department for pathology and forensic medicine, Zadar, Croatia

Fulltext: croatian, pdf (447 KB) pages 29-33 downloads: 1.360* cite
APA 6th Edition
Krvavica, A. (2009). P - 16 INK4a kao pokazatelj cervikalne displazije. Medica Jadertina, 39 (1-2), 29-33. Retrieved from
MLA 8th Edition
Krvavica, Ana. "P - 16 INK4a kao pokazatelj cervikalne displazije." Medica Jadertina, vol. 39, no. 1-2, 2009, pp. 29-33. Accessed 20 Sep. 2021.
Chicago 17th Edition
Krvavica, Ana. "P - 16 INK4a kao pokazatelj cervikalne displazije." Medica Jadertina 39, no. 1-2 (2009): 29-33.
Krvavica, A. (2009). 'P - 16 INK4a kao pokazatelj cervikalne displazije', Medica Jadertina, 39(1-2), pp. 29-33. Available at: (Accessed 20 September 2021)
Krvavica A. P - 16 INK4a kao pokazatelj cervikalne displazije. Medica Jadertina [Internet]. 2009 [cited 2021 September 20];39(1-2):29-33. Available from:
A. Krvavica, "P - 16 INK4a kao pokazatelj cervikalne displazije", Medica Jadertina, vol.39, no. 1-2, pp. 29-33, 2009. [Online]. Available: [Accessed: 20 September 2021]

In the period 2002-2004, 58 patients from Lozo Health Center were tested for HPV, 25 of them were HPV positive, 33 negative. 23 patients continued with medical treatment, while the others were excluded from treatment (went to other institutions or their follow-up forms had been lost).
After being prepared for immunohistochemical examination, all 23 tissue samples underwent retrograde immunohistochemical analysis with monoclonal mouse antibody p-16INK4a (DAKO Cytomation, Denmark) after antigen demasking and coupled with the DAKO En Vision TM visualisation system.
The results of immunohistochemical analysis were presented with semiquantitative score system from 0- 3. Four patients were p-16INK4a negative (1 with cervical polyp and 3 vulvar condylomata without dysplasia.). Three from seven patients with cytologic CIN I were p-16 negative, while the remaining four showed focally positive expression in the cytoplasm and nuclei of epithelial cells. 3 patients out of 6 with cytologically evident CIN II showed negative reaction with -16, 1 focally positive reaction, while two were strongly showing p-16INK4a. In 1 case from 3 patients with CIN III p-16 had negative expression, while the other two showed a strongly positive xpression. The only case with cytologic CIS had a diffuse and positive reaction with p-16INK4a. 1 from 2 patients with an unknown Papa-test result was diagnosed CIN IHPV negative), while the other was diagnosed CIN III on the cervical conus, and p-16INK4a expression was focally positive in the cytoplasm of epithelial cells.
From our results it is evident that p-16INK4a was positive in all tages of cervical dysplasia and clinically evident carcinoma CIS 69,5%),and HPV was confirmed with patients with CIN III. These two markers can therefore be used in prevention programmes with other currently known methods for they enable quick, simple and secure proof of the degree of dysplasia.

cervical intraepithelial neoplasia; cervical cancer; HPV; Papa-test; p-16

Hrčak ID: 37772



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