Izvorni znanstveni članak
Modeling Growth of Cellulomonas cellulans NRRL B 4567 under Substrate Inhibition During Cellulase Production
R. Agarwal
; Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati-781039, Assam, India
B. Mahanty
; Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati-781039, Assam, India
V. Venkata Dasu
; Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati-781039, Assam, India
Sažetak
Cellulase production study was performed in shake flask and bioreactor system using Cellulomonas cellulans NRRL B 4567 for initial substrate concentration from γS0 = 2 to 12 g L–1. The growth, substrate uptake profile and enzyme activity at different initial substrate concentrations were measured. The results inferred the presence of substrate inhibition kinetics. Various substrate inhibition models were tested and parameters were estimated, using non-linear regression analysis. Han-Levenspiel model was found
to be the best fitted model for both shake flask and reactor study. The highest volumetric enzyme activity was observed at initial substrate concentration of γS0 = 12 g L–1 and 4 g L–1 in shake flask and bioreactor respectively.
Ključne riječi
Cellulase production; substrate inhibition model; Cellulomonas cellulans
Hrčak ID:
38323
URI
Datum izdavanja:
30.6.2009.
Posjeta: 1.503 *