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https://doi.org/10.2478/10004-1254-60-2009-1895

D-Amphetamine Toxicity in Freshly Isolated Rat Hepatocytes: a Possible Role of CYP3A

Vessela Vitcheva ; Laboratory of Drug Metabolism and Drug Toxicity, Department of Pharmacology, Pharmacotherapy,and Toxicology, Faculty of Pharmacy, Medical University Sofia, Sofia, Bulgaria
Magdalena Kondeva-Burdina ; Laboratory of Drug Metabolism and Drug Toxicity, Department of Pharmacology, Pharmacotherapy, and Toxicology, Faculty of Pharmacy, Medical University Sofi a, Sofi a, Bulgaria
Mitka Mitcheva ; Laboratory of Drug Metabolism and Drug Toxicity, Department of Pharmacology, Pharmacotherapy, and Toxicology, Faculty of Pharmacy, Medical University Sofi a, Sofi a, Bulgaria

Puni tekst: engleski, pdf (87 KB) str. 139-145 preuzimanja: 664* citiraj
APA 6th Edition
Vitcheva, V., Kondeva-Burdina, M. i Mitcheva, M. (2009). D-Amphetamine Toxicity in Freshly Isolated Rat Hepatocytes: a Possible Role of CYP3A. Arhiv za higijenu rada i toksikologiju, 60 (2), 139-145. https://doi.org/10.2478/10004-1254-60-2009-1895
MLA 8th Edition
Vitcheva, Vessela, et al. "D-Amphetamine Toxicity in Freshly Isolated Rat Hepatocytes: a Possible Role of CYP3A." Arhiv za higijenu rada i toksikologiju, vol. 60, br. 2, 2009, str. 139-145. https://doi.org/10.2478/10004-1254-60-2009-1895. Citirano 30.09.2020.
Chicago 17th Edition
Vitcheva, Vessela, Magdalena Kondeva-Burdina i Mitka Mitcheva. "D-Amphetamine Toxicity in Freshly Isolated Rat Hepatocytes: a Possible Role of CYP3A." Arhiv za higijenu rada i toksikologiju 60, br. 2 (2009): 139-145. https://doi.org/10.2478/10004-1254-60-2009-1895
Harvard
Vitcheva, V., Kondeva-Burdina, M., i Mitcheva, M. (2009). 'D-Amphetamine Toxicity in Freshly Isolated Rat Hepatocytes: a Possible Role of CYP3A', Arhiv za higijenu rada i toksikologiju, 60(2), str. 139-145. https://doi.org/10.2478/10004-1254-60-2009-1895
Vancouver
Vitcheva V, Kondeva-Burdina M, Mitcheva M. D-Amphetamine Toxicity in Freshly Isolated Rat Hepatocytes: a Possible Role of CYP3A. Arh Hig Rada Toksikol. [Internet]. 2009 [pristupljeno 30.09.2020.];60(2):139-145. https://doi.org/10.2478/10004-1254-60-2009-1895
IEEE
V. Vitcheva, M. Kondeva-Burdina i M. Mitcheva, "D-Amphetamine Toxicity in Freshly Isolated Rat Hepatocytes: a Possible Role of CYP3A", Arhiv za higijenu rada i toksikologiju, vol.60, br. 2, str. 139-145, 2009. [Online]. https://doi.org/10.2478/10004-1254-60-2009-1895

Sažetak
The aim of this study was to trace D-amphetamine toxicity in isolated rat hepatocytes and to elucidate a possible involvement of CYP3A in the mechanisms of its toxicity. To this end, male Wistar rats were treated with nifedipine (5 mg kg-1 i.p., 5 days), a substrate and inducer of CYP3A. Hepatocytes isolated from nifedipine-treated and control rats were incubated with D-amphetamine at a concentration of 100 µmol L-1, which was determined to be an average toxic concentration (TC50) for the compound. To evaluate the possible toxic effects of D-amphetamine on freshly isolated rat hepatocytes, we assessed the following parameters: cell viability, lactate dehydrogenase (LDH) activity, and glutathione (GSH). The results showed that nifedipine potentiated amphetamine cytotoxicity in vitro, as follows: cell viability dropped by 65 % (p<0.001), GSH by 80 % (p<0.001), and LDH activity increased by 190 % (p<0.001). To clarify the role of nifedipine in amphetamine cytotoxicity, we used amiodarone, a substrate and an inhibitor of CYP3A. Pre-incubation of nifedipine-treated hepatocytes with amiodarone (14 µmol L-1) significantly lowered amphetamine cytotoxicity. Our results confirmed the toxicity of D-amphetamine in isolated rat hepatocytes and the involvement of CYP3A in its metabolism and hepatotoxicity.

Ključne riječi
amiodarone; amphetamine cytotoxicity; nifedipine

Hrčak ID: 38380

URI
https://hrcak.srce.hr/38380

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Posjeta: 1.021 *