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Izvorni znanstveni članak
https://doi.org/10.2478/10004-1254-60-2009-1917

Usporedba djelovanja blokatora kalcijevih kanala, blokatora autonomnoga živčanog sustava te inhibitora slobodnih radikala na hiposekreciju inzulin iz izolirnih langerhansovih otočića štakora uzrokovanu diazinonom

Nazila Pourkhalili ; Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
Shirin Pournourmohammadi ; Institute of Medicinal Plants, ACECR, Tehran, Iran
Fatemeh Rahimi ; Faculty of Pharmacy, and Pharmaceutical Sciences Research Center, Tehran University, of Medical Sciences, Tehran, Iran
Sanaz Vosough-Ghanbari ; Faculty of Pharmacy, and Pharmaceutical Sciences Research Center, Tehran University, of Medical Sciences, Tehran, Iran
Maryam Baeeri ; Faculty of Pharmacy, and Pharmaceutical Sciences Research Center, Tehran University, of Medical Sciences, Tehran, Iran
Seyed Nasser Ostad ; Faculty of Pharmacy, and Pharmaceutical Sciences Research Center, Tehran University, of Medical Sciences, Tehran, Iran
Mohammad Abdollahi ; Faculty of Pharmacy, and Pharmaceutical Sciences Research Center, Tehran University, of Medical Sciences, Tehran, Iran

Puni tekst: engleski, pdf (168 KB) str. 157-164 preuzimanja: 863* citiraj
APA 6th Edition
Pourkhalili, N., Pournourmohammadi, S., Rahimi, F., Vosough-Ghanbari, S., Baeeri, M., Ostad, S.N. i Abdollahi, M. (2009). Comparative Effects of Calcium Channel Blockers, Autonomic Nervous System Blockers, and Free Radical Scavengers on Diazinon-Induced Hyposecretion of Insulin from Isolated Islets of Langerhans in Rats. Arhiv za higijenu rada i toksikologiju, 60 (2), 157-164. https://doi.org/10.2478/10004-1254-60-2009-1917
MLA 8th Edition
Pourkhalili, Nazila, et al. "Comparative Effects of Calcium Channel Blockers, Autonomic Nervous System Blockers, and Free Radical Scavengers on Diazinon-Induced Hyposecretion of Insulin from Isolated Islets of Langerhans in Rats." Arhiv za higijenu rada i toksikologiju, vol. 60, br. 2, 2009, str. 157-164. https://doi.org/10.2478/10004-1254-60-2009-1917. Citirano 20.09.2020.
Chicago 17th Edition
Pourkhalili, Nazila, Shirin Pournourmohammadi, Fatemeh Rahimi, Sanaz Vosough-Ghanbari, Maryam Baeeri, Seyed Nasser Ostad i Mohammad Abdollahi. "Comparative Effects of Calcium Channel Blockers, Autonomic Nervous System Blockers, and Free Radical Scavengers on Diazinon-Induced Hyposecretion of Insulin from Isolated Islets of Langerhans in Rats." Arhiv za higijenu rada i toksikologiju 60, br. 2 (2009): 157-164. https://doi.org/10.2478/10004-1254-60-2009-1917
Harvard
Pourkhalili, N., et al. (2009). 'Comparative Effects of Calcium Channel Blockers, Autonomic Nervous System Blockers, and Free Radical Scavengers on Diazinon-Induced Hyposecretion of Insulin from Isolated Islets of Langerhans in Rats', Arhiv za higijenu rada i toksikologiju, 60(2), str. 157-164. https://doi.org/10.2478/10004-1254-60-2009-1917
Vancouver
Pourkhalili N, Pournourmohammadi S, Rahimi F, Vosough-Ghanbari S, Baeeri M, Ostad SN i sur. Comparative Effects of Calcium Channel Blockers, Autonomic Nervous System Blockers, and Free Radical Scavengers on Diazinon-Induced Hyposecretion of Insulin from Isolated Islets of Langerhans in Rats. Arh Hig Rada Toksikol. [Internet]. 2009 [pristupljeno 20.09.2020.];60(2):157-164. https://doi.org/10.2478/10004-1254-60-2009-1917
IEEE
N. Pourkhalili, et al., "Comparative Effects of Calcium Channel Blockers, Autonomic Nervous System Blockers, and Free Radical Scavengers on Diazinon-Induced Hyposecretion of Insulin from Isolated Islets of Langerhans in Rats", Arhiv za higijenu rada i toksikologiju, vol.60, br. 2, str. 157-164, 2009. [Online]. https://doi.org/10.2478/10004-1254-60-2009-1917

Sažetak
U osoba izloženih organofosfatnim insekticidima zamijećen je nastanak hiperglikemije. Svrha je ovo istraživanja bila usporediti djelovanje blokatora kalcijevih kanala, alfa i beta-adrenergičkih i muskarinskih receptora te inhibicije slobodnih radikala na lučenje inzulina iz Langerhansovih otočića izoliranih iz štakora tretiranih diazinonom. Otočići su izolirani iz gušterače štakora s pomoću standardnog postupka digestije kolagenazom, odvajanja centrifugiranjem i metodom ručnog probira (engl. hand-picking) te su kultivirani u inkubatoru pri 37 °C i 5 % CO2. Pokusne su kulture inkubirane s 1 mL glukoze u koncentraciji od 8 mmol L-1 te diazinonom u dozi od 125 μg mL-1, odnosno 625 μg mL-1. U kontrolu je dodana samo glukoza u koncentraciji od 8 mmol L-1. Nakon toga je u kulture dodan jedan od sljedećih agenasa: 30 µmol L-1 atropin, 100 µmol L-1 ACh + 10 µmol L-1 neostigmin, 0,1 µmol L-1 propranolol, 2 µmol L-1 nifedipin, 50 µmol L-1 fenoksibenzamin, odnosno 10 µmol L-1 alfa-tokoferol. U svim je pokusima diazinon značajno smanjio lučenje inzulina, s time da je doza od 125 μg mL-1 dovela do 62,2 %-tne inhibicije, a doza od 625 μg mL-1 do 64,38 %-tne inhibicije lučenja inzulina, što upućuje na djelovanje neovisno o dozi. Acetilkolin i alfa-tokoferol su ponovno potaknuli lučenje inzulina, za razliku od atropina koji ga je dodatno smanjio. Primjena blokatora alfa i beta-adrenergičkih receptora te blokatora kalcijevih kanala nije utjecala na djelovanje diazinona. Autori zaključuju da diazinon utječe na lučenje inzulina ponajviše narušavanjem ravnoteže između slobodnih radikala i antioksidansa u Langerhansovim otočićima te dovodi do toksičnoga stresa.

Ključne riječi
gušterača; oksidativni stres; organofosfati; štakori

Hrčak ID: 38391

URI
https://hrcak.srce.hr/38391

[engleski]

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