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A Novel Three-stage Process for Continuous Production of Penicillin G Acylase by a Temperature - sensitive Expression System of Bacillus subtilis Phagephi 105

Xin-Ai Chen
Pei-Lin Cen


Puni tekst: engleski pdf 240 Kb

str. 367-372

preuzimanja: 468

citiraj


Sažetak

This study is pertaining to the production of penicillin G acylase (PGA) by Bacillus subtilis 8105MU331 in which PGA gene, under the control of thermal-induced promoter, was integrated. The key process parameters including induced-temperature, induced- time, and culture temperature were optimized in flask culture. A three-stage cultivation process was developed for PGA production with the expression system of B. subtilis 8105MU331. Furthermore, a bioreactor with a thermal-induced apparatus was designed for continuous production of PGA, where cell growth, induction, and PGA expression could be conducted separately. At a dilution rate of 0.20 h–1, PGA production was taken under continuous cultivation in three-stage process. After continuous feeding, the cell density, pH, and residual glucose in the first- and third-reactor were maintained
steady for up to 40 h. These results suggested that the new three-stage process might be feasible and very efficient for production of heterologous proteins.

Ključne riječi

Penicillin G acylase; Bacillus subtilis; prophage; three-stage cultivation process

Hrčak ID:

3634

URI

https://hrcak.srce.hr/3634

Datum izdavanja:

20.12.2005.

Posjeta: 988 *