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Modulation of Osteogenic Cell Morphology by ECM Ligands and Enamel Matrix Derivative

Christoph Reichert ; Rheinische Friedrich-Wilhelms-University, Department of Orthodontics, Germany
Marcus Klein ; Johannes Gutenberg-University Mainz, Department of Oral and Maxillofacial Surgery, Germany
Adrian Kasaj ; Johannes Gutenberg-University Mainz, Department of Operative Dentistry and Periodontology, Germany
Sebastian Kuhn ; Johannes Gutenberg University, Department of Trauma Surgery, Germany
Hermann Götz ; Johannes Gutenberg-University Mainz, Institute of Applied Structure- and Microanalysis, Germany
Gerhard Hommel ; Johannes Gutenberg-University Mainz, Institute of Medical Biostatistics, Epidemiology and Informatics, Germany
Heinz Duschner ; Johannes Gutenberg-University Mainz, Institute of Applied Structure- and Microanalysis, Germany
Bilal Al-Nawas ; Johannes Gutenberg-University Mainz, Department of Oral and Maxillofacial Surgery, Germany

Puni tekst: engleski, pdf (761 KB) str. 188-201 preuzimanja: 406* citiraj
APA 6th Edition
Reichert, C., Klein, M., Kasaj, A., Kuhn, S., Götz, H., Hommel, G., ... Al-Nawas, B. (2009). Modulation of Osteogenic Cell Morphology by ECM Ligands and Enamel Matrix Derivative. Acta stomatologica Croatica, 43 (3), 188-201. Preuzeto s https://hrcak.srce.hr/40777
MLA 8th Edition
Reichert, Christoph, et al. "Modulation of Osteogenic Cell Morphology by ECM Ligands and Enamel Matrix Derivative." Acta stomatologica Croatica, vol. 43, br. 3, 2009, str. 188-201. https://hrcak.srce.hr/40777. Citirano 18.09.2020.
Chicago 17th Edition
Reichert, Christoph, Marcus Klein, Adrian Kasaj, Sebastian Kuhn, Hermann Götz, Gerhard Hommel, Heinz Duschner i Bilal Al-Nawas. "Modulation of Osteogenic Cell Morphology by ECM Ligands and Enamel Matrix Derivative." Acta stomatologica Croatica 43, br. 3 (2009): 188-201. https://hrcak.srce.hr/40777
Harvard
Reichert, C., et al. (2009). 'Modulation of Osteogenic Cell Morphology by ECM Ligands and Enamel Matrix Derivative', Acta stomatologica Croatica, 43(3), str. 188-201. Preuzeto s: https://hrcak.srce.hr/40777 (Datum pristupa: 18.09.2020.)
Vancouver
Reichert C, Klein M, Kasaj A, Kuhn S, Götz H, Hommel G i sur. Modulation of Osteogenic Cell Morphology by ECM Ligands and Enamel Matrix Derivative. Acta stomatologica Croatica [Internet]. 2009 [pristupljeno 18.09.2020.];43(3):188-201. Dostupno na: https://hrcak.srce.hr/40777
IEEE
C. Reichert, et al., "Modulation of Osteogenic Cell Morphology by ECM Ligands and Enamel Matrix Derivative", Acta stomatologica Croatica, vol.43, br. 3, str. 188-201, 2009. [Online]. Dostupno na: https://hrcak.srce.hr/40777. [Citirano: 18.09.2020.]
Puni tekst: hrvatski, pdf (761 KB) str. 188-201 preuzimanja: 364* citiraj
APA 6th Edition
Reichert, C., Klein, M., Kasaj, A., Kuhn, S., Götz, H., Hommel, G., ... Al-Nawas, B. (2009). Modulacija morfologije osteogenih stanica pomoću ECM liganada i derivata caklinskog matriksa. Acta stomatologica Croatica, 43 (3), 188-201. Preuzeto s https://hrcak.srce.hr/40777
MLA 8th Edition
Reichert, Christoph, et al. "Modulacija morfologije osteogenih stanica pomoću ECM liganada i derivata caklinskog matriksa." Acta stomatologica Croatica, vol. 43, br. 3, 2009, str. 188-201. https://hrcak.srce.hr/40777. Citirano 18.09.2020.
Chicago 17th Edition
Reichert, Christoph, Marcus Klein, Adrian Kasaj, Sebastian Kuhn, Hermann Götz, Gerhard Hommel, Heinz Duschner i Bilal Al-Nawas. "Modulacija morfologije osteogenih stanica pomoću ECM liganada i derivata caklinskog matriksa." Acta stomatologica Croatica 43, br. 3 (2009): 188-201. https://hrcak.srce.hr/40777
Harvard
Reichert, C., et al. (2009). 'Modulacija morfologije osteogenih stanica pomoću ECM liganada i derivata caklinskog matriksa', Acta stomatologica Croatica, 43(3), str. 188-201. Preuzeto s: https://hrcak.srce.hr/40777 (Datum pristupa: 18.09.2020.)
Vancouver
Reichert C, Klein M, Kasaj A, Kuhn S, Götz H, Hommel G i sur. Modulacija morfologije osteogenih stanica pomoću ECM liganada i derivata caklinskog matriksa. Acta stomatologica Croatica [Internet]. 2009 [pristupljeno 18.09.2020.];43(3):188-201. Dostupno na: https://hrcak.srce.hr/40777
IEEE
C. Reichert, et al., "Modulacija morfologije osteogenih stanica pomoću ECM liganada i derivata caklinskog matriksa", Acta stomatologica Croatica, vol.43, br. 3, str. 188-201, 2009. [Online]. Dostupno na: https://hrcak.srce.hr/40777. [Citirano: 18.09.2020.]

Sažetak
The precondition for successful periodontal regeneration is adequate activation of relevant cell populations like osteogenic cells. Here, cell adhesion and maturation are closely associated with cell morphology and f-actin cytoskeletal organisation. The potential of solitaire extracellular matrix (ECM) components as well as enamel matrix derivative (EMD) to enhance periodontal healing is well documented. Objective: The aim of the study was to test the impact of the ECM proteins collagen type 1 and laminin-1 as well as commercially available EMD on osteogenic cell morphology and cytoskeletal organisation. Material and methods: In an observational study, a total of 2450 osteogenic cells of 5 different cell lines (4 primary ones and 1 commercial one) cultivated on the respective substrates were analysed by 3 independent observers. After staining for the f-actin cytoskeleton and automated CLSM visualisation, cells were assigned to 3 different categories depending on morphological cell attributes (immature vs. intermediate vs. mature). Besides descriptive analysis, a multivariate logistic regression was performed to identify relevant influence parameters on cell morphology and cytoskeletal organisation. Results: The applied solitaire ligands collagen and laminin and especially EMD promoted a mature osteogenic phenotype. Nevertheless, considerable differences between the investigated cell lines could be identified as well. Analysis of cell morphology and cytoskeletal organisation offers a reliable method of acquiring the first hints of biocompatibility and bio-activation on different substrates. Conclusion: Our results highlight the potential of the investigated ligands to support periodontal regeneration by enhancing osteogenic cell attachment and maturation.

Ključne riječi
Osteoblasts; Extracellular Matrix; Enamel Matrix Proteins; Cytoskeleton; Periodontal Diseases

Hrčak ID: 40777

URI
https://hrcak.srce.hr/40777

[hrvatski]

Posjeta: 1.232 *