Izvorni znanstveni članak
Modulation of Osteogenic Cell Morphology by ECM Ligands and Enamel Matrix Derivative
Christoph Reichert
; Ortodontska klinika Sveučilišta Friedrich-Wilhelm, Njemačka
Marcus Klein
; Oralna i maksilofacijalna klinika Sveučilišta Johannes Gutenberg, Mainz, Njemačka
Adrian Kasaj
; Kirurška i parodontološka klinika Sveučilišta Johannes Gutenberg, Mainz, Njemačka
Sebastian Kuhn
; Traumatološka klinika Sveučilišta Johannes Gutenberg, Mainz, Njemačka
Hermann Götz
; Institut za mikroanalizu Sveučilišta Johannes Gutenberg, Mainz, Njemačka
Gerhard Hommel
; Institut za medicinsku biostatiku, epidemiologiju i informatiku (IMBEI) Sveučilišta Johannes Gutenberg, Mainz, Njemačka
Heinz Duschner
; Institut za mikroanalizu Sveučilišta Johannes Gutenberg, Mainz, Njemačka
Bilal Al-Nawas
; Oralna i maksilofacijalna klinika Sveučilišta Johannes Gutenberg, Mainz, Njemačka
Sažetak
The precondition for successful periodontal regeneration is adequate activation of relevant cell populations like osteogenic cells. Here, cell adhesion and maturation are closely associated with cell morphology and f-actin cytoskeletal organisation. The potential of solitaire extracellular matrix (ECM) components as well as enamel matrix derivative (EMD) to enhance periodontal healing is well documented. Objective: The aim of the study was to test the impact of the ECM proteins collagen type 1 and laminin-1 as well as commercially available EMD on osteogenic cell morphology and cytoskeletal organisation. Material and methods: In an observational study, a total of 2450 osteogenic cells of 5 different cell lines (4 primary ones and 1 commercial one) cultivated on the respective substrates were analysed by 3 independent observers. After staining for the f-actin cytoskeleton and automated CLSM visualisation, cells were assigned to 3 different categories depending on morphological cell attributes (immature vs. intermediate vs. mature). Besides descriptive analysis, a multivariate logistic regression was performed to identify relevant influence parameters on cell morphology and cytoskeletal organisation. Results: The applied solitaire ligands collagen and laminin and especially EMD promoted a mature osteogenic phenotype. Nevertheless, considerable differences between the investigated cell lines could be identified as well. Analysis of cell morphology and cytoskeletal organisation offers a reliable method of acquiring the first hints of biocompatibility and bio-activation on different substrates. Conclusion: Our results highlight the potential of the investigated ligands to support periodontal regeneration by enhancing osteogenic cell attachment and maturation.
Ključne riječi
Osteoblasts; Extracellular Matrix; Enamel Matrix Proteins; Cytoskeleton; Periodontal Diseases
Hrčak ID:
40777
URI
Datum izdavanja:
15.9.2009.
Posjeta: 2.255 *