APA 6th Edition MIO, Y. (2009). The beginning of human life under time-laps Cinematography. Periodicum biologorum, 111 (3), 323-327. Preuzeto s https://hrcak.srce.hr/43353
MLA 8th Edition MIO, YASUYUKI. "The beginning of human life under time-laps Cinematography." Periodicum biologorum, vol. 111, br. 3, 2009, str. 323-327. https://hrcak.srce.hr/43353. Citirano 08.08.2020.
Chicago 17th Edition MIO, YASUYUKI. "The beginning of human life under time-laps Cinematography." Periodicum biologorum 111, br. 3 (2009): 323-327. https://hrcak.srce.hr/43353
Harvard MIO, Y. (2009). 'The beginning of human life under time-laps Cinematography', Periodicum biologorum, 111(3), str. 323-327. Preuzeto s: https://hrcak.srce.hr/43353 (Datum pristupa: 08.08.2020.)
Vancouver MIO Y. The beginning of human life under time-laps Cinematography. Periodicum biologorum [Internet]. 2009 [pristupljeno 08.08.2020.];111(3):323-327. Dostupno na: https://hrcak.srce.hr/43353
IEEE Y. MIO, "The beginning of human life under time-laps Cinematography", Periodicum biologorum, vol.111, br. 3, str. 323-327, 2009. [Online]. Dostupno na: https://hrcak.srce.hr/43353. [Citirano: 08.08.2020.]
Sažetak Background and Purpose: The aim of this study was to follow and record the early stages of development of a human embryo using time-lapse cinematography, and to discuss the beginning of human life.
Materials and Methods: Human ova were incubated in thermo-stabilized and pH controlled chambers and observed using an inverted microscope fitted with a CCD digital camera. Images were taken at 2-minute intervals for 5–6 days. A total of 30 consecutive images, corresponding to an hour of incubation, could then be replayed in 1 second. This allowed us to watch the development of embryos over 5–6 days in a few minutes.
Results and Conclusion: We observed fertilization of an ovum with a
single spermatozoon followed by early cleavages, formation of the morula, blastocyst hatching and changes in the embryonic plates and the development of monozygotic twins in 2 of 3 incubated blastocysts. TLC has contributed enormously to our understanding of the morphological mechanisms of fertilization and embryonic development.We would like to suggest that the time at which stem cell-like morula cells form the differentiated blastocyst is the point at which human life begins, and that the ET of blastocyst may increase the risk of monozygotic twins.