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Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays

Tamara Braut ; Department of Otorhinolaryngology Head and Neck Surgery, Clinical Hospital Center Rijeka, Croatia
Mira Krstulja ; Department of Pathology, School of Medicine, University of Rijeka, Croatia
Milodar Kujundžić ; Department of Otorhinolaryngology Head and Neck Surgery, Clinical Hospital Center Rijeka, Croatia
Dubravko Manestar ; Department of Otorhinolaryngology Head and Neck Surgery, Clinical Hospital Center Rijeka, Croatia
Ita Hadžisejdić ; Department of Pathology, School of Medicine, University of Rijeka, Croatia
Nives Jonjić ; Department of Pathology, School of Medicine, University of Rijeka, Croatia
Blaženka Grahovac ; Department of Pathology, School of Medicine, University of Rijeka, Croatia
Darko Manestar ; Department of Otorhinolaryngology Head and Neck Surgery, Clinical Hospital Center Rijeka, Croatia

Puni tekst: engleski, pdf (271 KB) str. 370-379 preuzimanja: 392* citiraj
APA 6th Edition
Braut, T., Krstulja, M., Kujundžić, M., Manestar, D., Hadžisejdić, I., Jonjić, N., ... Manestar, D. (2009). Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays. Croatian medical journal, 50 (4), 370-379. Preuzeto s https://hrcak.srce.hr/47857
MLA 8th Edition
Braut, Tamara, et al. "Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays." Croatian medical journal, vol. 50, br. 4, 2009, str. 370-379. https://hrcak.srce.hr/47857. Citirano 15.11.2019.
Chicago 17th Edition
Braut, Tamara, Mira Krstulja, Milodar Kujundžić, Dubravko Manestar, Ita Hadžisejdić, Nives Jonjić, Blaženka Grahovac i Darko Manestar. "Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays." Croatian medical journal 50, br. 4 (2009): 370-379. https://hrcak.srce.hr/47857
Harvard
Braut, T., et al. (2009). 'Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays', Croatian medical journal, 50(4), str. 370-379. Preuzeto s: https://hrcak.srce.hr/47857 (Datum pristupa: 15.11.2019.)
Vancouver
Braut T, Krstulja M, Kujundžić M, Manestar D, Hadžisejdić I, Jonjić N i sur. Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays. Croat Med J. [Internet]. 2009 [pristupljeno 15.11.2019.];50(4):370-379. Dostupno na: https://hrcak.srce.hr/47857
IEEE
T. Braut, et al., "Epidermal Growth Factor Receptor Protein Expression and Gene Amplification in Normal, Hyperplastic, and Cancerous Glottic Tissue: Immunohistochemical and Fluorescent in Situ Hybridization Study on Tissue Microarrays", Croatian medical journal, vol.50, br. 4, str. 370-379, 2009. [Online]. Dostupno na: https://hrcak.srce.hr/47857. [Citirano: 15.11.2019.]

Sažetak
Aim To evaluate the importance of epidermal growth factor receptor
(EGFR) protein overexpression and gene amplification in
carcinogenesis of glottic cancer.
Method In order to evaluate EGFR expression at protein and
gene level, immunohistochemical (IHC) analysis and fluorescent
in situ hybridization (FISH) were performed on tissue microarrays
of laryngeal tissue (145 samples) – 38 samples of normal mucosa,
46 samples of hyperplastic lesions, and 61 samples of cancerous
lesions.
Results Membranous (mEGFR) and cytoplasmic (cEGFR) EGFR expression
was significantly different between the analyzed groups.
The differences were most striking in the suprabasal-transforming
zone. IHC evaluation showed that high and low mEGFR staining
contributed to the differentiation of dysplastic lesions, simple
hyperplasia, and cancerous tissue, as well as between different
degrees of atypia in hyperplastic lesions (P < 0.050). EGFR gene
amplification was not found in simple and abnormal hyperplastic
lesions, but it was confirmed in 2/21 atypical hyperplasias, indicating
that gene amplification can facilitate identification of
malignant potential in hyperplastic lesions. In cancerous tissue,
EGFR gene amplification was found in 8/50 samples. EGFR gene
amplification was found in preinvasive cancer in one patient. In
invasive carcinomas, gene amplification was not associated with
stage or grade. Carcinomas with gene amplification showed significantly
higher cEGFR expression (basal layer P = 0.003; suprabasal
layer P = 0.002).
Conclusions This study confirmed an increase in EGFR protein
expression and gene amplification with the increase in biological
aggressiveness of glottic lesions. A correlation between EGFR
gene amplification and protein expression was established. Gene
amplification proved to be an early event in glottic carcinogenesis,
indicating its importance for glottic cancer prevention, early
detection, and protocol selection.

Ključne riječi
EGFR; quantitative immunohistochemistry; FISH; glottic cancer; tissue microarray; EGFR-targeted therapies

Hrčak ID: 47857

URI
https://hrcak.srce.hr/47857

Posjeta: 598 *