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Enhanced delivery of etoposide to Dalton’s lymphoma in mice through polysorbate 20 micelles

LAKKIREDDY HARIVARDHAN REDDY
RAKESH KUMAR SHARMA
RAYASA RAMACHANDRA MURTHY

Puni tekst: engleski, pdf (128 KB) str. 143-155 preuzimanja: 573* citiraj
APA 6th Edition
REDDY, L.H., SHARMA, R.K. i MURTHY, R.R. (2006). Enhanced delivery of etoposide to Dalton’s lymphoma in mice through polysorbate 20 micelles. Acta Pharmaceutica, 56 (2), 143-155. Preuzeto s https://hrcak.srce.hr/4549
MLA 8th Edition
REDDY, LAKKIREDDY HARIVARDHAN, et al. "Enhanced delivery of etoposide to Dalton’s lymphoma in mice through polysorbate 20 micelles." Acta Pharmaceutica, vol. 56, br. 2, 2006, str. 143-155. https://hrcak.srce.hr/4549. Citirano 17.06.2019.
Chicago 17th Edition
REDDY, LAKKIREDDY HARIVARDHAN, RAKESH KUMAR SHARMA i RAYASA RAMACHANDRA MURTHY. "Enhanced delivery of etoposide to Dalton’s lymphoma in mice through polysorbate 20 micelles." Acta Pharmaceutica 56, br. 2 (2006): 143-155. https://hrcak.srce.hr/4549
Harvard
REDDY, L.H., SHARMA, R.K., i MURTHY, R.R. (2006). 'Enhanced delivery of etoposide to Dalton’s lymphoma in mice through polysorbate 20 micelles', Acta Pharmaceutica, 56(2), str. 143-155. Preuzeto s: https://hrcak.srce.hr/4549 (Datum pristupa: 17.06.2019.)
Vancouver
REDDY LH, SHARMA RK, MURTHY RR. Enhanced delivery of etoposide to Dalton’s lymphoma in mice through polysorbate 20 micelles. Acta Pharm. [Internet]. 2006 [pristupljeno 17.06.2019.];56(2):143-155. Dostupno na: https://hrcak.srce.hr/4549
IEEE
L.H. REDDY, R.K. SHARMA i R.R. MURTHY, "Enhanced delivery of etoposide to Dalton’s lymphoma in mice through polysorbate 20 micelles", Acta Pharmaceutica, vol.56, br. 2, str. 143-155, 2006. [Online]. Dostupno na: https://hrcak.srce.hr/4549. [Citirano: 17.06.2019.]

Sažetak
The study evaluates the possibility of enhancing uptake of etoposide (topoisomerase II inhibitor) by tumor when delivered through Polysorbate 20 micelles. The micelle formation was ascertained by determining the critical micellar concentration (CMC) with a du Nouy ring tensiometer and by size measurement using dynamic light scattering. Addition of 5% ethanol decreased the CMC of Polysorbate 20 (from 5.0 x 10-5 to 4.54 x 10-5 mol L-1). Etoposide (ET) and etoposide loaded Polysorbate 20 micelles (EPM) were radiolabeled with 99mTc by the reduction method using stannous chloride. Labeling parameters were optimized to obtain high labeling efficiency. The diethylenetriamine pentaacetic acid and cysteine challenge tests showed very low transchelation of 99mTc-ET and 99mTc-EPM complexes indicating their in vitro stability. The complexes also exhibited serum stability assessed by ascending thin layer chromatography. Subcutaneous injection of EPM resulted in significantly higher tumor uptake (~ 100 folds compared to ET 6 h post injection) (p < 0.001) and prolonged tumor retention. Tumor uptake was also confirmed by gamma imaging studies. EPM exhibited relatively high brain concentrations (~7 fold 24 h post injection) compared to ET, suggesting the potential use of EPM in the treatment of brain malignancies.

Ključne riječi
etoposide; polysorbate micelles; drug delivery; radiolabeling; biodistribution; tumor transport

Hrčak ID: 4549

URI
https://hrcak.srce.hr/4549

[hrvatski]

Posjeta: 914 *