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Acta Pharmaceutica, Vol. 60 No. 2, 2010.

Izvorni znanstveni članak
https://doi.org/10.2478/v10007-010-0012-0

Selektivna i osjetljiva metoda određivanja metoprolola u humanoj plazmi tekućinskom kromatografijom spregnutom s masenom spektrometrijom

Padala Venkateswarlu ; Analytical Chemistry Division, Department of Chemistry, Sri Venkateswara University,
Bommana Naresh Kumar ; Analytical Chemistry Division, Department of Chemistry, Sri Venkateswara University,
Kalluru Seshaiah ; Analytical Chemistry Division, Department of Chemistry, Sri Venkateswara University,
Vasireddy Veera Vara Prasad ; Bio-Analytical Division, Vimta Labs Limited, Cherlapally, Hyderabad-500051, India

Puni tekst: engleski, pdf (86 KB) str. 177-184 preuzimanja: 965* citiraj
APA 6th Edition
Venkateswarlu, P., Kumar, B.N., Seshaiah, K. i Prasad, V.V.V. (2010). Selective and sensitive method for the determination of metoprolol in human plasma using liquid chromatography coupled with tandem mass spectrometry. Acta Pharmaceutica, 60 (2), 177-184. https://doi.org/10.2478/v10007-010-0012-0
MLA 8th Edition
Venkateswarlu, Padala, et al. "Selective and sensitive method for the determination of metoprolol in human plasma using liquid chromatography coupled with tandem mass spectrometry." Acta Pharmaceutica, vol. 60, br. 2, 2010, str. 177-184. https://doi.org/10.2478/v10007-010-0012-0. Citirano 19.05.2019.
Chicago 17th Edition
Venkateswarlu, Padala, Bommana Naresh Kumar, Kalluru Seshaiah i Vasireddy Veera Vara Prasad. "Selective and sensitive method for the determination of metoprolol in human plasma using liquid chromatography coupled with tandem mass spectrometry." Acta Pharmaceutica 60, br. 2 (2010): 177-184. https://doi.org/10.2478/v10007-010-0012-0
Harvard
Venkateswarlu, P., et al. (2010). 'Selective and sensitive method for the determination of metoprolol in human plasma using liquid chromatography coupled with tandem mass spectrometry', Acta Pharmaceutica, 60(2), str. 177-184. doi: https://doi.org/10.2478/v10007-010-0012-0
Vancouver
Venkateswarlu P, Kumar BN, Seshaiah K, Prasad VVV. Selective and sensitive method for the determination of metoprolol in human plasma using liquid chromatography coupled with tandem mass spectrometry. Acta Pharm. [Internet]. 2010 [pristupljeno 19.05.2019.];60(2):177-184. doi: https://doi.org/10.2478/v10007-010-0012-0
IEEE
P. Venkateswarlu, B.N. Kumar, K. Seshaiah i V.V.V. Prasad, "Selective and sensitive method for the determination of metoprolol in human plasma using liquid chromatography coupled with tandem mass spectrometry", Acta Pharmaceutica, vol.60, br. 2, str. 177-184, 2010. [Online]. doi: https://doi.org/10.2478/v10007-010-0012-0

Sažetak
U radu je opisano određivanje metoprolola u humanoj plazmi tekućinskom kromatografijom visoke učinkovitosti spregnutom s masenom spektrometrijom. Analit i interni standard, nevirapin, ekstrahirani su iz plazme etil-acetatom. Kromatografsko odjeljivanje provedeno je na C-18 koloni uz mobilnu fazu sljedećeg sastava: 10 mmol L–1 amonijev acetat (pH 5,0)/acetonitril 15:85 (V/V). Ionizacija uzoraka provedena je pri atmosferskom tlaku. Povećanje selektivnosti postignuto je spregnutom masenom spektrometrijskom analizom praćenjem dvaju prijelaza (MRM) – 268,2 → 116,2 za metoprolol, odnosno 267,1 → 226,2 za nevirapin. Metoda je validirana za koncentracijsko područje 1200 ng mL–1 uz preciznost i točnost od 0,9 do 8,8 %, odnosno 89,9 do 105,8 %.

Ključne riječi
metoprolol; tekućinska kromatografija; spregnuta masena spektrometrija; humana plazma; bioekvivalencija

Hrčak ID: 48387

URI
https://hrcak.srce.hr/48387

[engleski]

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