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Izvorni znanstveni članak
https://doi.org/10.2478/v10007-010-0012-0

Selective and sensitive method for the determination of metoprolol in human plasma using liquid chromatography coupled with tandem mass spectrometry

Padala Venkateswarlu ; Analytical Chemistry Division, Department of Chemistry, Sri Venkateswara University,
Bommana Naresh Kumar ; Analytical Chemistry Division, Department of Chemistry, Sri Venkateswara University,
Kalluru Seshaiah ; Analytical Chemistry Division, Department of Chemistry, Sri Venkateswara University,
Vasireddy Veera Vara Prasad ; Bio-Analytical Division, Vimta Labs Limited, Cherlapally, Hyderabad-500051, India

Puni tekst: engleski, pdf (86 KB) str. 177-184 preuzimanja: 985* citiraj
APA 6th Edition
Venkateswarlu, P., Kumar, B.N., Seshaiah, K. i Prasad, V.V.V. (2010). Selective and sensitive method for the determination of metoprolol in human plasma using liquid chromatography coupled with tandem mass spectrometry. Acta Pharmaceutica, 60 (2), 177-184. https://doi.org/10.2478/v10007-010-0012-0
MLA 8th Edition
Venkateswarlu, Padala, et al. "Selective and sensitive method for the determination of metoprolol in human plasma using liquid chromatography coupled with tandem mass spectrometry." Acta Pharmaceutica, vol. 60, br. 2, 2010, str. 177-184. https://doi.org/10.2478/v10007-010-0012-0. Citirano 17.06.2019.
Chicago 17th Edition
Venkateswarlu, Padala, Bommana Naresh Kumar, Kalluru Seshaiah i Vasireddy Veera Vara Prasad. "Selective and sensitive method for the determination of metoprolol in human plasma using liquid chromatography coupled with tandem mass spectrometry." Acta Pharmaceutica 60, br. 2 (2010): 177-184. https://doi.org/10.2478/v10007-010-0012-0
Harvard
Venkateswarlu, P., et al. (2010). 'Selective and sensitive method for the determination of metoprolol in human plasma using liquid chromatography coupled with tandem mass spectrometry', Acta Pharmaceutica, 60(2), str. 177-184. doi: https://doi.org/10.2478/v10007-010-0012-0
Vancouver
Venkateswarlu P, Kumar BN, Seshaiah K, Prasad VVV. Selective and sensitive method for the determination of metoprolol in human plasma using liquid chromatography coupled with tandem mass spectrometry. Acta Pharm. [Internet]. 2010 [pristupljeno 17.06.2019.];60(2):177-184. doi: https://doi.org/10.2478/v10007-010-0012-0
IEEE
P. Venkateswarlu, B.N. Kumar, K. Seshaiah i V.V.V. Prasad, "Selective and sensitive method for the determination of metoprolol in human plasma using liquid chromatography coupled with tandem mass spectrometry", Acta Pharmaceutica, vol.60, br. 2, str. 177-184, 2010. [Online]. doi: https://doi.org/10.2478/v10007-010-0012-0

Sažetak
A high-performance liquid chromatography-tandem mass spectrometric method was developed and validated for the determination of metoprolol in human plasma. The analyte and internal standard, nevirapine,were extracted from plasma matrix by liquid–liquid extraction with ethyl acetate. Cromatographic separation was achieved on a C-18 analytical column with an isocratic mobile phase of 15:85 (V/V) 10 m mol L–1 ammonium acetate (pH 5.0)/acetonitrile. The atmospheric pressure chemical ionization technique was used for sample ionization in positive ion mode and enhanced selectivity was achieved by tandem mass spectrometric analysis via two multiple reaction monitoring (MRM) transitions, 268.2 → 116.2 for metoprolol and 267.1 → 226.2 for nevirapine, respectively. The assay was validated for human plasma over a concentration range of 1200 ng mL–1 with the precision and accuracy ranging from 0.9 to 8.8 % and 89.9 to 105.8 %, respectively.

Ključne riječi
metoprolol; liquid chromatography; tandem mass spectrometry; human plasma; bioequivalence

Hrčak ID: 48387

URI
https://hrcak.srce.hr/48387

[hrvatski]

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