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Acta Pharmaceutica, Vol. 60 No. 2, 2010.

Kratko priopćenje
https://doi.org/10.2478/v10007-010-0013-z

Validated RP-HPLC and TLC methods for simultaneous estimation of tamsulosin hydrochloride and finasteride in combined dosage forms

Dipti B. Patel ; S. K. Patel College of Pharmaceutical Education and Research, Department of Pharmaceutical Chemistry, Ganpat University, Kherva, Mehsana, Gujarat, India-382711
Natubhai J. Patel ; S. K. Patel College of Pharmaceutical Education and Research, Department of Pharmaceutical Chemistry, Ganpat University, Kherva, Mehsana, Gujarat, India-382711

Puni tekst: engleski, pdf (101 KB) str. 197-205 preuzimanja: 1.356* citiraj
APA 6th Edition
Patel, D.B. i Patel, N.J. (2010). Validated RP-HPLC and TLC methods for simultaneous estimation of tamsulosin hydrochloride and finasteride in combined dosage forms. Acta Pharmaceutica, 60 (2), 197-205. https://doi.org/10.2478/v10007-010-0013-z
MLA 8th Edition
Patel, Dipti B. i Natubhai J. Patel. "Validated RP-HPLC and TLC methods for simultaneous estimation of tamsulosin hydrochloride and finasteride in combined dosage forms." Acta Pharmaceutica, vol. 60, br. 2, 2010, str. 197-205. https://doi.org/10.2478/v10007-010-0013-z. Citirano 16.02.2019.
Chicago 17th Edition
Patel, Dipti B. i Natubhai J. Patel. "Validated RP-HPLC and TLC methods for simultaneous estimation of tamsulosin hydrochloride and finasteride in combined dosage forms." Acta Pharmaceutica 60, br. 2 (2010): 197-205. https://doi.org/10.2478/v10007-010-0013-z
Harvard
Patel, D.B., i Patel, N.J. (2010). 'Validated RP-HPLC and TLC methods for simultaneous estimation of tamsulosin hydrochloride and finasteride in combined dosage forms', Acta Pharmaceutica, 60(2), str. 197-205. doi: https://doi.org/10.2478/v10007-010-0013-z
Vancouver
Patel DB, Patel NJ. Validated RP-HPLC and TLC methods for simultaneous estimation of tamsulosin hydrochloride and finasteride in combined dosage forms. Acta Pharm. [Internet]. 2010 [pristupljeno 16.02.2019.];60(2):197-205. doi: https://doi.org/10.2478/v10007-010-0013-z
IEEE
D.B. Patel i N.J. Patel, "Validated RP-HPLC and TLC methods for simultaneous estimation of tamsulosin hydrochloride and finasteride in combined dosage forms", Acta Pharmaceutica, vol.60, br. 2, str. 197-205, 2010. [Online]. doi: https://doi.org/10.2478/v10007-010-0013-z

Sažetak
Reversed phase high-performance liquid chromatography (RP-HPLC) and thin-layer chromatography (TLC) methods have been developed and validated for simultaneous estimation of tamsulosin hydrochloride and finasteride in bulk drug and in combined dosage forms. RP-HPLC separation was achieved on a Phenomenex C18 column using methanol/0.02 mol L-1 ammonium acetate buffer/triethylamine (79.9 + 20 + 0.1, V/V/V) (pH 9.2) as mobile phase. The TLC separation was achieved on an aluminium-backed layer of silica gel 60F254 using toluene/methanol/triethylamine (9 + 1.5 + 1, V/V/V) as eluent. Quantification was achieved with photodiode array (PDA) detection at 235 nm over the concentration range 0.5–16 and 150 µg mL1 with mean recovery of 99.8 ± 0.9 and 100.0 ± 0.8 % for tamsulosin hydrochloride and finasteride, respectively, by the RP-HPLC method. Quantification was achieved with UV detection at 270 nm over the concentration range 100–2000 ng per spot and 250–5000 ng per spot with mean recovery of 98.9 ± 0.9 and 99.6 ± 0.7 % for tamsulosin hydrochloride and finasteride, respectively, by the TLC method. Both methods are simple, precise, accurate and sensitive and are applicable to the simultaneous determination of tamsulosin hydrochloride and finasteride in bulk drug and in combined dosage forms.

Ključne riječi
tamsulosin hydrochloride; finasteride; RP-HPLC; TLC

Hrčak ID: 48388

URI
https://hrcak.srce.hr/48388

[hrvatski]

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