Izvorni znanstveni članak
Production of a Fermented Solid Containing Lipases of Rhizopus microsporus and Its Application in the Pre-Hydrolysis of a High-Fat Dairy Wastewater
Dayane Alberton
; Department of Biochemistry and Molecular Biology, Federal University of Paraná, Centro Politécnico, P.O. Box 19046, Curitiba 81531-980, Paraná, Brazil
David Alexander Mitchell
; Department of Biochemistry and Molecular Biology, Federal University of Paraná, Centro Politécnico, P.O. Box 19046, Curitiba 81531-980, Paraná, Brazil
Jesús Cordova
; Department of Chemical Engineering, CUCEI, University of Guadalajara, Blvd. Marcelino García Barragán y Calz. Olímpica, 44840 Guadalajara, Jalisco, Mexico
Patrício Peralta-Zamora
; Department of Chemistry, Federal University of Paraná, Centro Politécnico, P.O. Box 19081, Curitiba 81531-980, Paraná, Brazil
Nadia Krieger
; Department of Chemistry, Federal University of Paraná, Centro Politécnico, P.O. Box 19081, Curitiba 81531-980, Paraná, Brazil
Sažetak
The filamentous fungus Rhizopus microsporus CPQBA 312-07 DRM was grown in solid-state cultivation and the fermented solid produced was used to hydrolyze triacylglycerols in a high-fat dairy wastewater. For the solid-state cultivation, a mixture of sunflower seed meal and sugarcane bagasse (1:3 by mass on dry basis) was selected. After 18 h of culture, the fermented product had an activity, measured titrimetrically against triolein, of 26 U per gram of dry solids. This substrate mixture does not suffer from compaction and therefore can be used in large scale solid-state cultivation bioreactors. When used to pretreat a high-fat dairy wastewater, with an oil and grease level above 1300 mg/L, the fermented solid reduced the oil and grease level to below 300 mg/L after 72 h at 35 °C. Further work is required to improve the production of lipolytic activity in the solid-state cultivation step and to find the optimum pretreatment time in the wastewater pretreatment step.
Ključne riječi
lipolysis; enzymatic pretreatment; solid-state fermentation; solid-state cultivation; dairy wastewater; Rhizopus microsporus
Hrčak ID:
48432
URI
Datum izdavanja:
5.3.2010.
Posjeta: 1.555 *