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Original scientific paper

iglD plays a crucial role in intracellular replication of F. tularensis in A. castellanii

Martin Brezovec ; Department for microbiology and parasitology, School of medicine, University of Rijeka, Rijeka, Croatia
Aida Iljazović ; Department for microbiology and parasitology, School of medicine, University of Rijeka, Rijeka, Croatia
Zlata Zaharija ; Department for microbiology and parasitology, School of medicine, University of Rijeka, Rijeka, Croatia
Mateja Ožanić ; Department for microbiology and parasitology, School of medicine, University of Rijeka, Rijeka, Croatia
Marina Šantić ; Department for microbiology and parasitology, School of medicine, University of Rijeka, Rijeka, Croatia

Fulltext: croatian, pdf (2 MB) pages 65-68 downloads: 475* cite
APA 6th Edition
Brezovec, M., Iljazović, A., Zaharija, Z., Ožanić, M. & Šantić, M. (2010). iglD ima važnu ulogu u unutarstaničnom razmnožavanju F. tularensis u A. castellanii. Medicina Fluminensis, 46 (1), 65-68. Retrieved from https://hrcak.srce.hr/48840
MLA 8th Edition
Brezovec, Martin, et al. "iglD ima važnu ulogu u unutarstaničnom razmnožavanju F. tularensis u A. castellanii." Medicina Fluminensis, vol. 46, no. 1, 2010, pp. 65-68. https://hrcak.srce.hr/48840. Accessed 12 May 2021.
Chicago 17th Edition
Brezovec, Martin, Aida Iljazović, Zlata Zaharija, Mateja Ožanić and Marina Šantić. "iglD ima važnu ulogu u unutarstaničnom razmnožavanju F. tularensis u A. castellanii." Medicina Fluminensis 46, no. 1 (2010): 65-68. https://hrcak.srce.hr/48840
Harvard
Brezovec, M., et al. (2010). 'iglD ima važnu ulogu u unutarstaničnom razmnožavanju F. tularensis u A. castellanii', Medicina Fluminensis, 46(1), pp. 65-68. Available at: https://hrcak.srce.hr/48840 (Accessed 12 May 2021)
Vancouver
Brezovec M, Iljazović A, Zaharija Z, Ožanić M, Šantić M. iglD ima važnu ulogu u unutarstaničnom razmnožavanju F. tularensis u A. castellanii. Medicina Fluminensis [Internet]. 2010 [cited 2021 May 12];46(1):65-68. Available from: https://hrcak.srce.hr/48840
IEEE
M. Brezovec, A. Iljazović, Z. Zaharija, M. Ožanić and M. Šantić, "iglD ima važnu ulogu u unutarstaničnom razmnožavanju F. tularensis u A. castellanii", Medicina Fluminensis, vol.46, no. 1, pp. 65-68, 2010. [Online]. Available: https://hrcak.srce.hr/48840. [Accessed: 12 May 2021]

Abstracts
Introduction: Francisella tularensis is a gram-negative facultative intracellular bacterium that can cause a fatal disease, tularemia, in human and animals. This organism has been isolated from over 250 wildlife species, including fish, birds, amphibians, rabbits, squirrels, hares, voles, ticks, and flies. It resists harsh environments, and has been shown to survive in water and mud for more than a year. The natural reservoirs in water are protozoa including many species of amoeba. Previous results have shown survival and growth of F. tularensis LVS strain in Acanthamoeba castellanii.
Methods: In this work we tested the intracellular replication of F. tularensis subsp. novicida and mutant strains iglD by CFU and confocal microscopy.
Results: There is no evidence about the role of IglD protein in intracellular survival and replication of F. tularensis subsp. novicida inside A. castellanii. Our results show that F. tularensis subsp. novicida replicates in A. castellanii cells with the peak of infection at 48 hours. The iglD mutant showed growth defect in A. castellanii.
Discussion: This data are consistent with previous finding in mammalian cells where this gene is crucial for intracellular replication of F. tularensis subsp. novicida in macrophages cells.
Conclusion: Our results pointed the similarities between two different environmental niche for F. tularensis survival and pathogenicity.

Keywords
Acanthamoeba castellanii; Francisella tularensis; iglD mutant

Hrčak ID: 48840

URI
https://hrcak.srce.hr/48840

[croatian]

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