Izvorni znanstveni članak
https://doi.org/10.2478/10004-1254-61-2010-1967
Assessment of Cyto/Genotoxicity of Irinotecan in V79 Cells Using the Comet, Micronucleus, and Chromosome Aberration Assay
Vilena Kašuba
; Institut za medicinska istraživanja i medicinu rada, Zagreb
Ružica Rozgaj
; Institut za medicinska istraživanja i medicinu rada, Zagreb
Marija Gamulin
; Clinical Hospital Centre Zagreb, Zagreb
Ivančica Trošić
; Institut za medicinska istraživanja i medicinu rada, Zagreb
Sažetak
Irinotecan is a topoisomerase I interactive agent, widely used in the treatment of metastatic colorectal cancer. The genotoxic effects of the maximum single dose (18 μg mL-1), recommended monotherapy dose (9 μg mL-1), and recommended combined therapy dose (4.5 μg mL-1) of irinotecan were studied on V79 cells using the comet assay, chromosome aberration assay, and micronucleus test. The cells were treated with irinotecan for 2 h or 24 h. The statistical signifi cance of the results was determined using the one-way ANOVA test and a nonparametric Mann Whitney U test. The comet assay did not show dose-dependent or time-dependent effects. The chromosome aberration analysis showed large DNA rearrangements, i.e.,
chromosome exchanges. Although the exposed cultures showed a signifi cant increase in micronucleated cells in respect to control, no dose-dependent relation was established among the treated cultures. Timedependent effect was also not observed.
Ključne riječi
alkaline comet assay; antineoplastic drug; cell cultures; DNA and cytogenetic damage; topoisomerase
Hrčak ID:
49011
URI
Datum izdavanja:
17.3.2010.
Posjeta: 1.834 *