Izvorni znanstveni članak
https://doi.org/10.2478/10004-1254-61-2010-1988
Cage Exposure of European Sea Bass (Dicentrarchus labrax) for In Situ Assessment of Pollution-Related Genotoxicity
Maja Šrut
; Department of Zoology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Anamarija Štambuk
; Department of Zoology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Mirjana Pavlica
; Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Göran I. V. Klobučar
; Department of Zoology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Sažetak
Genotoxic effects are often the earliest signs of pollution-related environmental disturbance. In this study, we used the comet assay and micronucleus test to assess DNA damage in the erythrocytes of the European sea bass (Dicentrarchus labrax) exposed to environmental pollution in situ. Fish were collected from a
fi sh farm in the Trogir Bay and their cages placed at an unpolluted reference site Šolta (Nečujam Bay) and a polluted site Vranjic (Kaštela Bay) for four weeks. A group of fi sh which remained at the fi sh farm Trogir Bay were used as the second control group. Fish exposed at the Vranjic site showed a signifi cantly higher erythrocyte DNA damage, measured by the comet assay, than either control group. Micronucleus induction showed a similar gradient of DNA damage, but did not reach statistical signifi cance. Our results show that cage exposure of a marine fi sh D. labrax can be useful in environmental biomonitoring and confi rm the comet assay as a suitable tool for detecting pollution-related genotoxicity.
Ključne riječi
Adriatic Sea; comet assay; fish; ecogenotoxicology; marine biomonitoring; micronucleus test
Hrčak ID:
49014
URI
Datum izdavanja:
17.3.2010.
Posjeta: 1.922 *