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Original scientific paper

Separation of Racemic Mixtures of Amino Acids Using Chiral Eluents

P. Dimitrova ; TU Kaiserslautern, Thermische Verfahrenstechnik, P.O. Box 3049; 67653 Kaiserslautern, Germany
H.-J. Bart ; TU Kaiserslautern, Thermische Verfahrenstechnik, P.O. Box 3049; 67653 Kaiserslautern, Germany

Fulltext: english, pdf (226 KB) pages 75-83 downloads: 1.116* cite
APA 6th Edition
Dimitrova, P. & Bart, H.-. (2010). Separation of Racemic Mixtures of Amino Acids Using Chiral Eluents. Chemical and Biochemical Engineering Quarterly, 24 (1), 75-83. Retrieved from https://hrcak.srce.hr/49484
MLA 8th Edition
Dimitrova, P. and H.-J. Bart. "Separation of Racemic Mixtures of Amino Acids Using Chiral Eluents." Chemical and Biochemical Engineering Quarterly, vol. 24, no. 1, 2010, pp. 75-83. https://hrcak.srce.hr/49484. Accessed 19 Sep. 2019.
Chicago 17th Edition
Dimitrova, P. and H.-J. Bart. "Separation of Racemic Mixtures of Amino Acids Using Chiral Eluents." Chemical and Biochemical Engineering Quarterly 24, no. 1 (2010): 75-83. https://hrcak.srce.hr/49484
Harvard
Dimitrova, P., and Bart, H.-. (2010). 'Separation of Racemic Mixtures of Amino Acids Using Chiral Eluents', Chemical and Biochemical Engineering Quarterly, 24(1), pp. 75-83. Available at: https://hrcak.srce.hr/49484 (Accessed 19 September 2019)
Vancouver
Dimitrova P, Bart H-. Separation of Racemic Mixtures of Amino Acids Using Chiral Eluents. Chemical and Biochemical Engineering Quarterly [Internet]. 2010 [cited 2019 September 19];24(1):75-83. Available from: https://hrcak.srce.hr/49484
IEEE
P. Dimitrova and H.-. Bart, "Separation of Racemic Mixtures of Amino Acids Using Chiral Eluents", Chemical and Biochemical Engineering Quarterly, vol.24, no. 1, pp. 75-83, 2010. [Online]. Available: https://hrcak.srce.hr/49484. [Accessed: 19 September 2019]

Abstracts
A commercially available non-chiral RP-C8 chromatographic column is used with an aqueous chiral mobile phase containing N,N-dimethyl-L-phenylalanine and copper in a ratio 20 : 1 for the enantiomeric resolution of the following amino acids: DL-valine (Val), DL-methionine (Met), DL-leucine (Leu), DL-phenylalanine (Phe) and DL-tyrosine (Tyr). The influence of organic modifier, temperature and flow-rate on the capacity factors and on the selectivity is experimentally determined and the separation behavior is
discussed. The capacity factors of some of the amino acids remained constant with changing the composition of the mobile phase, i.e. increasing methanol amount. With increase of the temperature the retention times decreased and the selectivities were slightly
reduced. In a study of the effect on retention and selectivity, linear van’t Hoff plots were obtained giving ∆H and ∆S values of the solute transfer from the mobile to the stationary phase. These were found to be within the range obtained for hydrophobic compounds in RP-HPLC systems. Using van Deemter plots high separation efficiency and low apparent axial dispersion coefficients for all amino acids could be determined.

Keywords
Amino acid; chiral mobile phase; ligand exchange chromatography; van Deemter plot; van’t Hoff plot

Hrčak ID: 49484

URI
https://hrcak.srce.hr/49484

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