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Response Surface Methodology for Optimization of the Erythromycin Production by Fed-Batch Fermentation Using an Inexpensive Biological Nitrogen Source

X. Zou ; State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, P. R. China; College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, P. R. China
C.-F. Chen ; State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, P. R. China
H.-F. Hang ; State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, P. R. China
J. Chu ; State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, P. R. China
Y.-P. Zhuang ; State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, P. R. China
S.-L. Zhang ; State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology, Shanghai 200237, P. R. China

Puni tekst: engleski, pdf (363 KB) str. 95-100 preuzimanja: 756* citiraj
APA 6th Edition
Zou, X., Chen, C.-., Hang, H.-., Chu, J., Zhuang, Y.-. i Zhang, S.-. (2010). Response Surface Methodology for Optimization of the Erythromycin Production by Fed-Batch Fermentation Using an Inexpensive Biological Nitrogen Source. Chemical and Biochemical Engineering Quarterly, 24 (1), 95-100. Preuzeto s https://hrcak.srce.hr/49490
MLA 8th Edition
Zou, X., et al. "Response Surface Methodology for Optimization of the Erythromycin Production by Fed-Batch Fermentation Using an Inexpensive Biological Nitrogen Source." Chemical and Biochemical Engineering Quarterly, vol. 24, br. 1, 2010, str. 95-100. https://hrcak.srce.hr/49490. Citirano 25.08.2019.
Chicago 17th Edition
Zou, X., C.-F. Chen, H.-F. Hang, J. Chu, Y.-P. Zhuang i S.-L. Zhang. "Response Surface Methodology for Optimization of the Erythromycin Production by Fed-Batch Fermentation Using an Inexpensive Biological Nitrogen Source." Chemical and Biochemical Engineering Quarterly 24, br. 1 (2010): 95-100. https://hrcak.srce.hr/49490
Harvard
Zou, X., et al. (2010). 'Response Surface Methodology for Optimization of the Erythromycin Production by Fed-Batch Fermentation Using an Inexpensive Biological Nitrogen Source', Chemical and Biochemical Engineering Quarterly, 24(1), str. 95-100. Preuzeto s: https://hrcak.srce.hr/49490 (Datum pristupa: 25.08.2019.)
Vancouver
Zou X, Chen C-, Hang H-, Chu J, Zhuang Y-, Zhang S-. Response Surface Methodology for Optimization of the Erythromycin Production by Fed-Batch Fermentation Using an Inexpensive Biological Nitrogen Source. Chemical and Biochemical Engineering Quarterly [Internet]. 2010 [pristupljeno 25.08.2019.];24(1):95-100. Dostupno na: https://hrcak.srce.hr/49490
IEEE
X. Zou, C.-. Chen, H.-. Hang, J. Chu, Y.-. Zhuang i S.-. Zhang, "Response Surface Methodology for Optimization of the Erythromycin Production by Fed-Batch Fermentation Using an Inexpensive Biological Nitrogen Source", Chemical and Biochemical Engineering Quarterly, vol.24, br. 1, str. 95-100, 2010. [Online]. Dostupno na: https://hrcak.srce.hr/49490. [Citirano: 25.08.2019.]

Sažetak
A novel source of biological nitrogen produced by multi-strain fermentation was developed for erythromycin production.The nitrogen composition of corn steep liquor, biological nitrogen and soybean flour was optimized by using Response surface methodology (RSM) firstly. A Box-Behnken design was used to estimate the optimum nitrogen composition for the production of erythromycin as follows: corn steep liquor: γ =5.1 g L-1, biological nitrogen γ = 5.96 g L-1 and soybean flour γ = 24.17 g L-1.
Based on the optimum nitrogen source, the constant glucose fed-batch and pH control fed-batch strategies were used to further optimize the erythromycin production in 50 L stirred bioreactor, respectively.It was found that pH control could serve as an effective
control strategy for erythromycin production, and the maximum erythromycin production was 8528 U mL-1 at 190 h.This work demonstrates that the new medium formulation based on a cheap nitrogen source, biological nitrogen, is a potential alternative for
economic erythromycin production on a large scale.

Ključne riječi
Biological nitrogen; erythromycin production; fed-batch strategy; medium optimization; response surface methodology; Saccharopolyspora erythraea

Hrčak ID: 49490

URI
https://hrcak.srce.hr/49490

Posjeta: 1.033 *