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Digital Morphometry of Cytologic Aspirate Endometrial Samples

Vesna Mahovlić ; Department of Gynecologic Cytology, University Department of Gynecology and Obstetrics, University Hospital Center Zagreb, Zagreb, Croatia
Ana Ovanin-Rakić ; Department of Gynecologic Cytology, University Department of Gynecology and Obstetrics, University Hospital Center Zagreb, Zagreb, Croatia
Lada Škopljanac-Mačina ; Department of Gynecologic Cytology, University Department of Gynecology and Obstetrics, University Hospital Center Zagreb, Zagreb, Croatia
Ana Barišić ; Department of Gynecologic Cytology, University Department of Gynecology and Obstetrics, University Hospital Center Zagreb, Zagreb, Croatia
Sanda Rajhvajn ; Department of Gynecologic Cytology, University Department of Gynecology and Obstetrics, University Hospital Center Zagreb, Zagreb, Croatia
Danijela Jurič ; Department of Gynecologic Cytology, University Department of Gynecology and Obstetrics, University Hospital Center Zagreb, Zagreb, Croatia
Ivana Šamija Projić ; Department of Gynecologic Cytology, University Department of Gynecology and Obstetrics, University Hospital Center Zagreb, Zagreb, Croatia
Jadranka Ilić-Forko ; University of Zagreb, School of Medicine, Zagreb, Croatia
Damir Babić ; University of Zagreb, School of Medicine, Zagreb, Croatia
Snježana Škrablin-Kučić ; University of Zagreb, School of Medicine, Zagreb, Croatia
Jadranka Božikov ; University of Zagreb, School of Medicine, Zagreb, Croatia

Puni tekst: engleski, pdf (144 KB) str. 45-51 preuzimanja: 411* citiraj
APA 6th Edition
Mahovlić, V., Ovanin-Rakić, A., Škopljanac-Mačina, L., Barišić, A., Rajhvajn, S., Jurič, D., ... Božikov, J. (2010). Digital Morphometry of Cytologic Aspirate Endometrial Samples. Collegium antropologicum, 34 (1), 45-51. Preuzeto s https://hrcak.srce.hr/51240
MLA 8th Edition
Mahovlić, Vesna, et al. "Digital Morphometry of Cytologic Aspirate Endometrial Samples." Collegium antropologicum, vol. 34, br. 1, 2010, str. 45-51. https://hrcak.srce.hr/51240. Citirano 29.01.2020.
Chicago 17th Edition
Mahovlić, Vesna, Ana Ovanin-Rakić, Lada Škopljanac-Mačina, Ana Barišić, Sanda Rajhvajn, Danijela Jurič, Ivana Šamija Projić, et al. "Digital Morphometry of Cytologic Aspirate Endometrial Samples." Collegium antropologicum 34, br. 1 (2010): 45-51. https://hrcak.srce.hr/51240
Harvard
Mahovlić, V., et al. (2010). 'Digital Morphometry of Cytologic Aspirate Endometrial Samples', Collegium antropologicum, 34(1), str. 45-51. Preuzeto s: https://hrcak.srce.hr/51240 (Datum pristupa: 29.01.2020.)
Vancouver
Mahovlić V, Ovanin-Rakić A, Škopljanac-Mačina L, Barišić A, Rajhvajn S, Jurič D i sur. Digital Morphometry of Cytologic Aspirate Endometrial Samples. Collegium antropologicum [Internet]. 2010 [pristupljeno 29.01.2020.];34(1):45-51. Dostupno na: https://hrcak.srce.hr/51240
IEEE
V. Mahovlić, et al., "Digital Morphometry of Cytologic Aspirate Endometrial Samples", Collegium antropologicum, vol.34, br. 1, str. 45-51, 2010. [Online]. Dostupno na: https://hrcak.srce.hr/51240. [Citirano: 29.01.2020.]

Sažetak
Unlike cervical cytology, morphological cytology criteria in the differential diagnosis of endometrium have not yet been clearly defined, and methods to allow for more precise evaluation of endometrium status have been searched for. The aim of the present study was to assess the value of morphometric nucleus analysis of cytologic aspirate endometrial samples in proliferative, hyperplastic and malignant endometrium by use of digital image analysis. Morphometric analysis was performed on archival cytologic aspirate endometrial samples (at least 10 per group) stained according to Papanicolaou (n=77) and May-Grünwald-Giemsa (MGG; n=80) with the following histopathologic diagnoses: proliferative endometrium, hyperplasia simplex, hyperplasia complex, hyperplasia complex atypica, and adenocarcinoma endometriodes endometrii (grade I, II and III). Interactive image analysis (nuclear area, convex area, perimeter, maximum and minimum radius, length and breadth, as well as nucleus form factor and elongation factor) was performed by use of the Sform software (Vamstec, Zagreb) on at least 50 (Papanicolaou stain) and 100 (MGG stain) well preserved endometrial epithelial cell nuclei without overlapping, at magnification of ´1000. Statistical data analysis was done by use of the Statistica Ver. 6 statistical package. Multivariate analysis (ANOVA) distinguished malignant, hyperplastic and proliferative endometrium according to all morphometric variables with both staining methods (p<0.05). However, on interactive testing of the groups (Kruskal-Wallis test), hyperplasias without atypia yielded no significant differences (p>0.05) from atypical hyperplasia, adenocarcinoma and proliferative endometrium only according to the nucleus form factor and elongation factor (Papanicolaou stain), whereas malignant and atypical hyperplastic endometrium (MGG stain) differed statistically significantly (p<0.05) from proliferative and hyperplastic endometrium without atypia according to all study parameters except for the nucleus form factor (p>0.05). According to the cytologic staining method, morphometric parameters were considerably higher in MGG stained endometrial samples, reaching the level of statistical significance (p<0.05) except for the nucleus form factor and elongation factor (p>0.05) in the groups of hyperplasia simplex and complex, well differentiated adenocarcinoma (form factor) and atypical hyperplasia (elongation factor). A combination of cytomorphology and the morphometric variables assessed in this study can yield useful information on the cytologic state of endometrium, with special reference to the possible differentiation of the group of hyperplasia without atypia from the group of adenocarcinoma and atypical hyperplasia.

Ključne riječi
endometrial cytology; digital image analysis; morphometry

Hrčak ID: 51240

URI
https://hrcak.srce.hr/51240

Posjeta: 684 *