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External Quality Assessment in Clinical Cell Analysis by Flow Cytometry. Why is It so Important?

Zoran Šiftar ; Institute of Clinical Chemistry, University Hospital »Merkur«, Zagreb, Croatia
Mirjana Mariana Kardum Paro ; Institute of Clinical Chemistry, University Hospital »Merkur«, Zagreb, Croatia
Ivica Sokolić ; Institute of Clinical Chemistry, University Hospital »Merkur«, Zagreb, Croatia
Aida Nazor ; Institute of Clinical Chemistry, University Hospital »Merkur«, Zagreb, Croatia
Zlata Flegar Meštrić ; University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, Croatia

Puni tekst: engleski, pdf (2 MB) str. 207-217 preuzimanja: 729* citiraj
APA 6th Edition
Šiftar, Z., Kardum Paro, M.M., Sokolić, I., Nazor, A. i Flegar Meštrić, Z. (2010). External Quality Assessment in Clinical Cell Analysis by Flow Cytometry. Why is It so Important?. Collegium antropologicum, 34 (1), 207-217. Preuzeto s https://hrcak.srce.hr/51266
MLA 8th Edition
Šiftar, Zoran, et al. "External Quality Assessment in Clinical Cell Analysis by Flow Cytometry. Why is It so Important?." Collegium antropologicum, vol. 34, br. 1, 2010, str. 207-217. https://hrcak.srce.hr/51266. Citirano 10.05.2021.
Chicago 17th Edition
Šiftar, Zoran, Mirjana Mariana Kardum Paro, Ivica Sokolić, Aida Nazor i Zlata Flegar Meštrić. "External Quality Assessment in Clinical Cell Analysis by Flow Cytometry. Why is It so Important?." Collegium antropologicum 34, br. 1 (2010): 207-217. https://hrcak.srce.hr/51266
Harvard
Šiftar, Z., et al. (2010). 'External Quality Assessment in Clinical Cell Analysis by Flow Cytometry. Why is It so Important?', Collegium antropologicum, 34(1), str. 207-217. Preuzeto s: https://hrcak.srce.hr/51266 (Datum pristupa: 10.05.2021.)
Vancouver
Šiftar Z, Kardum Paro MM, Sokolić I, Nazor A, Flegar Meštrić Z. External Quality Assessment in Clinical Cell Analysis by Flow Cytometry. Why is It so Important?. Collegium antropologicum [Internet]. 2010 [pristupljeno 10.05.2021.];34(1):207-217. Dostupno na: https://hrcak.srce.hr/51266
IEEE
Z. Šiftar, M.M. Kardum Paro, I. Sokolić, A. Nazor i Z. Flegar Meštrić, "External Quality Assessment in Clinical Cell Analysis by Flow Cytometry. Why is It so Important?", Collegium antropologicum, vol.34, br. 1, str. 207-217, 2010. [Online]. Dostupno na: https://hrcak.srce.hr/51266. [Citirano: 10.05.2021.]

Sažetak
Participation in external quality assessment is an integral part of laboratory work and mandatory when the results have a clinical application, which is one of the requirements of standard 15189 for accreditation of medical laboratories. Institute of Clinical Chemistry, the first laboratory accredited for clinical cell analysis by flow cytometry in Croatia, participated in UKNEQAS for Leukocyte Immunophenotyping in 3 schemes: »Immune Monitoring«, »CD34 Stem Cell Enumeration« and »Leukaemia Immunophenotyping«. For sample processing on EPICS XL flow cytometer, lyse/no wash preparation technique with ammonium chloride (NH4Cl) or ImmunoPrep lysing reagent was employed. In »Immune monitoring« programme CD45/sideward light scatter (SSC) proposed gating strategy was adopted for lymphocyte subsets, while modified ISHAGE protocol was used for CD34+ cell enumeration. Absolute count determination was performed on flow cytometer using FlowCount beads solution. In the period from the beginning of 2006 until the middle of 2009 a total number of 100 stabilized whole blood samples were processed. The relative and absolute enumeration results for lymphocyte subsets were within tolerable limits, in 97.1 and 97.1% of cases, and 95 and 90% of CD34+ cell enumeration, respectively. In immune monitoring CD45/SSC proposed gating strategy is the most frequent analysis used (>85% participants) and ISHAGE protocol for CD34+ cell determination with continuous rise from 76 to 83%. A number of participants who accept beads method for absolute count enumeration on flow cytometer get greater, 69 to 86%, while FlowCount was the second of bead-based techniques used (25 and 35%). Sample treatment in lyse/no wash technique using NH4Cl lysing solution was dominant procedure used by more than 1/3 participants, although its home made solution has replaced slowly by commercial reagents. The unacceptable results, 6 of 244, were obtained for 20 most frequently determined cell antigens in »Leukaemia Immunophenotyping« samples screened for leukaemia/lymphoma. Processing results of all participants showed that the deviation from laboratory guidelines and the use of older methods for cell identification, quantification of cell counting on haematology analyser, or usage an antibody conjugated with fluorochrome lesser fluorescence quantum often lead to an unacceptable result, although is noticeable trend to accept new referrals and protocols to reduce the inter-laboratory differences.

Ključne riječi
external quality assessment; UKNEQAS for Leukocyte Immunophenotyping; laboratory guidelines

Hrčak ID: 51266

URI
https://hrcak.srce.hr/51266

Posjeta: 993 *