hrcak mascot   Srce   HID

Original scientific paper

Flow Cytometric Analysis of Deep-Seated Lymph Nodes

Zorana Miletić
Branimir Gizdić
Tajana Štoos-Veić
Gordana Kaić
Nina-Petra Novak
Mario Tadić
Ozren Jakšić
Karmen Trutin Ostović

Fulltext: english, pdf (136 KB) pages 377-380 downloads: 264* cite
APA 6th Edition
Miletić, Z., Gizdić, B., Štoos-Veić, T., Kaić, G., Novak, N., Tadić, M., ... Trutin Ostović, K. (2010). Flow Cytometric Analysis of Deep-Seated Lymph Nodes. Collegium antropologicum, 34 (2), 377-380. Retrieved from https://hrcak.srce.hr/56435
MLA 8th Edition
Miletić, Zorana, et al. "Flow Cytometric Analysis of Deep-Seated Lymph Nodes." Collegium antropologicum, vol. 34, no. 2, 2010, pp. 377-380. https://hrcak.srce.hr/56435. Accessed 16 Dec. 2019.
Chicago 17th Edition
Miletić, Zorana, Branimir Gizdić, Tajana Štoos-Veić, Gordana Kaić, Nina-Petra Novak, Mario Tadić, Ozren Jakšić and Karmen Trutin Ostović. "Flow Cytometric Analysis of Deep-Seated Lymph Nodes." Collegium antropologicum 34, no. 2 (2010): 377-380. https://hrcak.srce.hr/56435
Harvard
Miletić, Z., et al. (2010). 'Flow Cytometric Analysis of Deep-Seated Lymph Nodes', Collegium antropologicum, 34(2), pp. 377-380. Available at: https://hrcak.srce.hr/56435 (Accessed 16 December 2019)
Vancouver
Miletić Z, Gizdić B, Štoos-Veić T, Kaić G, Novak N, Tadić M, et al. Flow Cytometric Analysis of Deep-Seated Lymph Nodes. Collegium antropologicum [Internet]. 2010 [cited 2019 December 16];34(2):377-380. Available from: https://hrcak.srce.hr/56435
IEEE
Z. Miletić, et al., "Flow Cytometric Analysis of Deep-Seated Lymph Nodes", Collegium antropologicum, vol.34, no. 2, pp. 377-380, 2010. [Online]. Available: https://hrcak.srce.hr/56435. [Accessed: 16 December 2019]

Abstracts
Flow cytometry (FC) immunophenotyping is an important tool in the evaluation of lymphadenopathy and is widely used in the diagnosis of non-Hodgkin’s lymphomas (NHLs) on fine-needle aspirates of lymph nodes and extranodal sites. Because at least 80% of NHLs are of B-cell type, detection of immunoglobulin (Ig) light-chain-restriction is the most commonly used method for confirmation of monoclonality. The aim of our study was to evaluate usefulness of endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) for FC analysis from deep-seated lymph nodes and to compare results of FC clonality analysis to cytomorphologic diagnosis of sampled lymph nodes. For cytological diagnosis direct smears were made, selected slide was stained for rapid-on site evaluation procedure. Sixteen patients with suspected NHL of deep-seated lymph nodes obtained by EUS-FNA were submitted for FC clonality analysis using four-color multiparameter flow cytometry stained with kappa /lambda/CD19/CD45. Clonality analysis was performed on 11 samples. Monoclonality was demonstrated in seven of 11 cases cytologically diagnosed as NHL and four of 11 cases cytologically diagnosed as benign were polyclonal. Our results show that EUS-FNAC with FC is a sensitive and specific tool in the diagnosis of deep-seated B-NHL. Cytologic diagnosis combined with FC clonality analysis can be performed in majority of cases and may eliminate need for open biopsy in some cases.

Keywords
flow cytometry; fine-needle aspiration cytology; endoscopic ultrasound; lymph node

Hrčak ID: 56435

URI
https://hrcak.srce.hr/56435

Visits: 466 *