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Acta Pharmaceutica, Vol. 60 No. 4, 2010.

Izvorni znanstveni članak
https://doi.org/10.2478/v10007-010-0032-9

Sensitive and selective spectrophotometric assay of doxycycline hyclate in pharmaceuticals using Folin-Ciocalteu reagent

PAVAGADA JAGANNATHAMURTHY RAMESH ; Department of Studies in Chemistry, University of Mysore, Manasagangothri, India-570 006
KANAKAPURA BASAVAIAH ; Department of Studies in Chemistry, University of Mysore, Manasagangothri, India-570 006
NAGARAJU RAJENDRAPRASAD ; Department of Studies in Chemistry, University of Mysore, Manasagangothri, India-570 006

Puni tekst: engleski, pdf (77 KB) str. 445-454 preuzimanja: 1.335* citiraj
APA 6th Edition
RAMESH, P.J., BASAVAIAH, K. i RAJENDRAPRASAD, N. (2010). Sensitive and selective spectrophotometric assay of doxycycline hyclate in pharmaceuticals using Folin-Ciocalteu reagent. Acta Pharmaceutica, 60 (4), 445-454. https://doi.org/10.2478/v10007-010-0032-9
MLA 8th Edition
RAMESH, PAVAGADA JAGANNATHAMURTHY, et al. "Sensitive and selective spectrophotometric assay of doxycycline hyclate in pharmaceuticals using Folin-Ciocalteu reagent." Acta Pharmaceutica, vol. 60, br. 4, 2010, str. 445-454. https://doi.org/10.2478/v10007-010-0032-9. Citirano 19.02.2019.
Chicago 17th Edition
RAMESH, PAVAGADA JAGANNATHAMURTHY, KANAKAPURA BASAVAIAH i NAGARAJU RAJENDRAPRASAD. "Sensitive and selective spectrophotometric assay of doxycycline hyclate in pharmaceuticals using Folin-Ciocalteu reagent." Acta Pharmaceutica 60, br. 4 (2010): 445-454. https://doi.org/10.2478/v10007-010-0032-9
Harvard
RAMESH, P.J., BASAVAIAH, K., i RAJENDRAPRASAD, N. (2010). 'Sensitive and selective spectrophotometric assay of doxycycline hyclate in pharmaceuticals using Folin-Ciocalteu reagent', Acta Pharmaceutica, 60(4), str. 445-454. doi: https://doi.org/10.2478/v10007-010-0032-9
Vancouver
RAMESH PJ, BASAVAIAH K, RAJENDRAPRASAD N. Sensitive and selective spectrophotometric assay of doxycycline hyclate in pharmaceuticals using Folin-Ciocalteu reagent. Acta Pharm. [Internet]. 2010 [pristupljeno 19.02.2019.];60(4):445-454. doi: https://doi.org/10.2478/v10007-010-0032-9
IEEE
P.J. RAMESH, K. BASAVAIAH i N. RAJENDRAPRASAD, "Sensitive and selective spectrophotometric assay of doxycycline hyclate in pharmaceuticals using Folin-Ciocalteu reagent", Acta Pharmaceutica, vol.60, br. 4, str. 445-454, 2010. [Online]. doi: https://doi.org/10.2478/v10007-010-0032-9

Sažetak
A spectrophotometric method for the determination of doxycycline (DOX) is described. The method is based on the formation of blue colored chromogen due to reduction of tungstate and/or molybdate in Folin-Ciocalteu (F-C) reagent by DOX in alkaline medium. The colored species has an absorption maximum at 770 nm and the system obeys Beer’s law over the concentration range 0.7512.0 μg mL1 DOX. The apparent molar absorptivity is 2.78 × 104 L mol1 cm1. The limit of quantification and detection values are reported to be 0.20 and 0.08 µg mL1, respectively. Over the linear range applicable, the accuracy and precision of the method were evaluated on intra-day and inter-day basis. The reported mean accuracy value was 101.0 ± 1.7 %, the relative error was ≤ 2.7 % and the relative standard deviation was ≤ 2.5 %. Application of the proposed method to bulk powder and commercial pharmaceutical tablets is also presented. No significant difference was obtained between the results of the proposed method and the official BP method. The procedure described in this paper is simple, rapid, accurate and precise.

Ključne riječi
doxycycline hyclate; Folin-Ciocalteu reagent; spectrophotometry

Hrčak ID: 59304

URI
https://hrcak.srce.hr/59304

[hrvatski]

Posjeta: 1.596 *