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Insulin Administration in the Mild Hyperglycaemia Changes Expression of Proinflammatory Adhesion Molecules on Human Aortic Endothelial Cells

Tatjana Bačun
Ljubica Glavaš-Obrovac
Tatjana Belovari
Ivan Mihaljević
Toni Hanich
Vesna Feher Belaj
Aleksandar Včev


Puni tekst: engleski pdf 297 Kb

str. 911-915

preuzimanja: 491

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Sažetak

An overexpression of cell adhesion molecules (CAMs) on the surface of endothelial cells is one of the first steps in a high glucose-mediated endothelial dysfunction in diabetic patients. The effect of insulin administration in the condition of elevated glucose concentration on the E-selectin, intracellular adhesion molecule-1 (ICAM-1) and vascular adhesion molecule-1 (VCAM-1) expression on human aortic endothelial cells (HAEC) was investigated. Cells were cultured for 4h in a medium supplemented with homocysteine (7 mM) and different concentration of glucose (5.5, 8.0, 12.0 and 16.5 mM respectively) with or without insulin (1 mIU/mL) addition. Expression of CAMs was analysed by flow-cytometry using monoclonal antibodies. Controls were CAMs expression in the medium with a corresponding glucose concentration. Obtained results show that short-term exposure of HAECs to moderate high glucose concentrations results in increased expression of E-selectin (2-fold), VCAM-1 (3-fold) and ICAM-1 (47%). At the same time, HAEC grown with 12 mM glucose expressed lesser E-selectin and, more ICAM-1 (for 64%) and VCAM-1 (41%) molecules. 16.5 mM glucose decreased expression of all investigated adhesion molecules. Addition of insulin was not changed expression of CAMs in a medium with 5.5 mM glucose. In conditions of elevated glucose concentration (12 mM), addition of insulin significantly dropped E-selectin (27%) and increased VCAM-1 (23%) expression. In conclusion, moderate elevated glucose concentration increased expression of cell adhesion molecules on HAEC. Insulin administration in the mild hyperglycaemia reduces an expression of the proinflammatory adhesion molecule E-selectin which could contribute in deceleration of macrovascular complications development in diabetic patients.

Ključne riječi

cell adhesion molecules; mild hyperglycaemia; insulin; human aortic endothelial cells

Hrčak ID:

59332

URI

https://hrcak.srce.hr/59332

Datum izdavanja:

1.10.2010.

Posjeta: 1.162 *