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Seryl-tRNA Synthetases from Methanogenic Archaea: Suppression of Bacterial Amber Mutation and Heterologous Toxicity

Sonja Lesjak ; University of Zagreb, Faculty of Science, Department of Chemistry, Horvatovac 102a, HR-10000 Zagreb, Croatia
Drasko Boko ; University of Zagreb, Faculty of Science, Department of Chemistry, Horvatovac 102a, HR-10000 Zagreb, Croatia
Ivana Weygand-Durasevic ; University of Zagreb, Faculty of Science, Department of Chemistry, Horvatovac 102a, HR-10000 Zagreb, Croatia

Puni tekst: engleski, pdf (244 KB) str. 512-518 preuzimanja: 365* citiraj
APA 6th Edition
Lesjak, S., Boko, D. i Weygand-Durasevic, I. (2010). Seryl-tRNA Synthetases from Methanogenic Archaea: Suppression of Bacterial Amber Mutation and Heterologous Toxicity. Food Technology and Biotechnology, 48 (4), 512-518. Preuzeto s https://hrcak.srce.hr/61722
MLA 8th Edition
Lesjak, Sonja, et al. "Seryl-tRNA Synthetases from Methanogenic Archaea: Suppression of Bacterial Amber Mutation and Heterologous Toxicity." Food Technology and Biotechnology, vol. 48, br. 4, 2010, str. 512-518. https://hrcak.srce.hr/61722. Citirano 21.01.2020.
Chicago 17th Edition
Lesjak, Sonja, Drasko Boko i Ivana Weygand-Durasevic. "Seryl-tRNA Synthetases from Methanogenic Archaea: Suppression of Bacterial Amber Mutation and Heterologous Toxicity." Food Technology and Biotechnology 48, br. 4 (2010): 512-518. https://hrcak.srce.hr/61722
Harvard
Lesjak, S., Boko, D., i Weygand-Durasevic, I. (2010). 'Seryl-tRNA Synthetases from Methanogenic Archaea: Suppression of Bacterial Amber Mutation and Heterologous Toxicity', Food Technology and Biotechnology, 48(4), str. 512-518. Preuzeto s: https://hrcak.srce.hr/61722 (Datum pristupa: 21.01.2020.)
Vancouver
Lesjak S, Boko D, Weygand-Durasevic I. Seryl-tRNA Synthetases from Methanogenic Archaea: Suppression of Bacterial Amber Mutation and Heterologous Toxicity. Food Technology and Biotechnology [Internet]. 2010 [pristupljeno 21.01.2020.];48(4):512-518. Dostupno na: https://hrcak.srce.hr/61722
IEEE
S. Lesjak, D. Boko i I. Weygand-Durasevic, "Seryl-tRNA Synthetases from Methanogenic Archaea: Suppression of Bacterial Amber Mutation and Heterologous Toxicity", Food Technology and Biotechnology, vol.48, br. 4, str. 512-518, 2010. [Online]. Dostupno na: https://hrcak.srce.hr/61722. [Citirano: 21.01.2020.]

Sažetak
Methanogenic archaea possess unusual seryl-tRNA synthetases (SerRS), evolutionarily distinct from the SerRSs found in other archaea, eucaryotes and bacteria. Our recent X-ray structural analysis of Methanosarcina barkeri SerRS revealed an idiosyncratic N-terminal domain and catalytic zinc ion in the active site. To shed further light on substrate discrimination by methanogenic-type SerRS, we set up to explore in vivo the interaction of methanogenic-type SerRSs with their cognate tRNAs in Escherichia coli or Saccharomyces cerevisiae. The expression of various methanogenic-type SerRSs was toxic for E. coli, resulting in the synthesis of erroneous proteins, as revealed by β-galactosidase stability assay. Although SerRSs from methanogenic archaea recognize tRNAsSer from all three domains of life in vitro, the toxicity presumably precluded the complementation of endogenous SerRS function in both, E. coli and S. cerevisiae. However, despite the observed toxicity, coexpression of methanogenic-type SerRS with its cognate tRNA suppressed bacterial amber mutation.

Ključne riječi
seryl-tRNA synthetase; heterologous toxicity; amber suppression; methanogenic archaea

Hrčak ID: 61722

URI
https://hrcak.srce.hr/61722

[hrvatski]

Posjeta: 695 *