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Statistical Screening of Supplementary Nitrogen Source for Enhanced Production of L-Asparaginase by Aspergillus terreus 1782

G. Baskar ; Department of Biotechnology, St. Joseph’s College of Engineering, Chennai-600119, India
C. Sriharini ; Department of Biotechnology, St. Joseph’s College of Engineering, Chennai-600119, India
R. Sripriya ; Department of Biotechnology, St. Joseph’s College of Engineering, Chennai-600119, India
S. Renganathan ; Department of Chemical Engineering, Alagappa College of Technology, Anna University Chennai, Chennai-600 025, India


Puni tekst: engleski pdf 113 Kb

str. 467-472

preuzimanja: 397

citiraj


Sažetak

In the present work, Latin Square Design (LSD) was applied to find the best supplementary nitrogen source for extracellular L-asparaginase production by Aspergillus terreus MTCC 1782 using corn flour as substrate in submerged fermentation. The independent
effect of supplementary nitrogen source such as ammonium chloride, urea and sodium nitrate was studied on growth and production of L-asparaginase by A. terreus. Statistical significance of the nitrogen sources was studied by performing analysis of variance (ANOVA) and graphical ANOVA for LSD using Data plot software. It was found that there is no significant difference on growth due to the change in supplementary nitrogen source studied. Urea was identified as best supplementary nitrogen source with mean L-asparaginase production of 33.25 IU mL–1 and mean biomass production of 12.99 mg mL–1.

Ključne riječi

L-Asparaginase activity; submerged fermentation; latin square design; supplementary nitrogen source

Hrčak ID:

62717

URI

https://hrcak.srce.hr/62717

Datum izdavanja:

27.12.2010.

Posjeta: 913 *