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Proizvodnja limunske kiseline pomoću kvasaca uzgojenih na podlogama s glicerolom, dobivenim pri proizvodnji biodizela

Svetlana V. Kamzolova   ORCID icon orcid.org/0000-0003-4326-2345 ; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr-t Nauki 5, Pushchino, RU-142290 Moscow Region, Russia
Alina R. Fatykhova ; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr-t Nauki 5, Pushchino, RU-142290 Moscow Region, Russia
Emiliya G. Dedyukhina ; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr-t Nauki 5, Pushchino, RU-142290 Moscow Region, Russia
Savas G. Anastassiadis ; Pythia Institute of Biotechnology, Avgi, GR-57002 Thessaloniki, Greece
Nikolay P. Golovchenko ; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr-t Nauki 5, Pushchino, RU-142290 Moscow Region, Russia
Igor G. Morgunov ; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr-t Nauki 5, Pushchino, RU-142290 Moscow Region, Russia

Puni tekst: engleski, pdf (182 KB) str. 65-74 preuzimanja: 2.160* citiraj
APA 6th Edition
Kamzolova, S.V., Fatykhova, A.R., Dedyukhina, E.G., Anastassiadis, S.G., Golovchenko, N.P. i Morgunov, I.G. (2011). Citric Acid Production by Yeast Grown on Glycerol-Containing Waste from Biodiesel Industry. Food Technology and Biotechnology, 49 (1), 65-74. Preuzeto s https://hrcak.srce.hr/65579
MLA 8th Edition
Kamzolova, Svetlana V., et al. "Citric Acid Production by Yeast Grown on Glycerol-Containing Waste from Biodiesel Industry." Food Technology and Biotechnology, vol. 49, br. 1, 2011, str. 65-74. https://hrcak.srce.hr/65579. Citirano 19.10.2021.
Chicago 17th Edition
Kamzolova, Svetlana V., Alina R. Fatykhova, Emiliya G. Dedyukhina, Savas G. Anastassiadis, Nikolay P. Golovchenko i Igor G. Morgunov. "Citric Acid Production by Yeast Grown on Glycerol-Containing Waste from Biodiesel Industry." Food Technology and Biotechnology 49, br. 1 (2011): 65-74. https://hrcak.srce.hr/65579
Harvard
Kamzolova, S.V., et al. (2011). 'Citric Acid Production by Yeast Grown on Glycerol-Containing Waste from Biodiesel Industry', Food Technology and Biotechnology, 49(1), str. 65-74. Preuzeto s: https://hrcak.srce.hr/65579 (Datum pristupa: 19.10.2021.)
Vancouver
Kamzolova SV, Fatykhova AR, Dedyukhina EG, Anastassiadis SG, Golovchenko NP, Morgunov IG. Citric Acid Production by Yeast Grown on Glycerol-Containing Waste from Biodiesel Industry. Food Technology and Biotechnology [Internet]. 2011 [pristupljeno 19.10.2021.];49(1):65-74. Dostupno na: https://hrcak.srce.hr/65579
IEEE
S.V. Kamzolova, A.R. Fatykhova, E.G. Dedyukhina, S.G. Anastassiadis, N.P. Golovchenko i I.G. Morgunov, "Citric Acid Production by Yeast Grown on Glycerol-Containing Waste from Biodiesel Industry", Food Technology and Biotechnology, vol.49, br. 1, str. 65-74, 2011. [Online]. Dostupno na: https://hrcak.srce.hr/65579. [Citirano: 19.10.2021.]

Sažetak
Ispitana je mogućnost uporabe glicerola i otpada bogatoga glicerolom, dobivenih pri proizvodnji biodizela, kao izvora ugljika i energije za mikrobnu proizvodnju limunske kiseline. Prethodno je ispitana proizvodnja limunske kiseline na selektivnim podlogama pomoću 66 sojeva kvasaca različitih vrsta (Candida, Pichia, Saccharomyces, Torulopsis i Yarrowia). Pri smanjenim koncentracijama dušika 41 soj (uglavnom vrste Yarrowia lipolytica) mogao je proizvesti limunsku kiselinu, dok 25 sojeva vrsta Debaryomyces, Candida, Pichiа, Saccharomyces i Torulopsis to nije moglo. Od 41 soja odabran je mutant Yarrowia lipolytica N15, jer je mogao proizvesti veliku količinu limunske kiseline. Ispitan je utjecaj pH-vrijednosti podloge, prozračivanja tijekom reakcije i koncentracije glicerola u podlozi na proizvodnju limunske kiseline pomoću toga soja. U optimalnim uvjetima u fermentoru mutant Yarrowia lipolytica N15 proizveo je do 98 g/L limunske kiseline na podlozi sa čistim glicerolom, a do 71 g/L limunske kiseline na podlozi s otpadom bogatim glicerolom. U radu se raspravlja i o utjecaju faze rasta na fiziološke značajke kvasaca pri proizvodnji limunske kiseline.

Ključne riječi
Yarrowia lipolytica; proizvodnja limunske kiseline; proizvodnja biodizela; glicerol; otpad bogat glicerolom

Hrčak ID: 65579

URI
https://hrcak.srce.hr/65579

[engleski]

Posjeta: 2.891 *