cytoplasmic male sterility, pepper, pollen fertility, restorer gene specific marker, F2
Abstract
In the present study the molecular basis and mechanism of pepper cytoplasmic male sterility (CMS) and its restoration system (Rf) has been characterized in detail. Pollen fertility of five CMSs (No. 201 to 205), four restorer lines (No. 206, 207, 209 and 210) and their F1, F2 generations were investigated during different growing seasons to study the response of male sterility to various environmental conditions. Restorer gene specific primers were applied to reveal the molecular genetic differences between the CMS and restorer lines.
„Bulk segregant” and individual analysis screened DNA markers linked to the fertility restorer (Rf) gene for cytoplasmic male sterility.
By the application for conventional breeding and molecular genetic methods co-segregation of the restorer specific markers and pollen viability data were observed on hundred individuals of the F2 generation in order to construct a physical linkage map.
