chlorophyll, drought stress, inoculation, nodulation, proline, soybean, glycine max l., stress index
Abstract
During the experiments were monitored the following varieties: Impala (South African Republic), Maverick (USA), Rankoshi No.1h (Japan) and their reactions to the water supply at the beginning of the growth stage R1 and R2 – blooming in an interaction with the Nitrazon inoculation of the seeds before a sowing. Mentioned genetic resources were provided for our research purposes by the Plant Production Research Center (PRRC) Pieštany, Gene Bank of the Slovak Republic. Seeding of the particular soy genotypes was made into the containers whilst 50% of seed corn from each genotype was before the sowing inoculated by the usage of Nitrazon inoculant (supplier: Agrokomp, spol. s.r.o., Modra). Water stress was secured by an irrigation interruption for a 7-day period in the mentioned growth stage. This stress had a negative impact on the relative water content in RWC plants, proline content, osmotic potential, SPAD, stress index as well as an amount of nodules on the roots by the all monitored varieties. According to an evaluation of the mentioned indicators more significant proline accumulation was confirmed by the genotype Maverick especially by the variant exposed to water deficit (3,25 µmol*g-1 FW according to the calculations on 100% RWC) without the inoculant Nitrazon use and inoculating variant 2.99 µmol*g-1 FW according to calculations on 100% RWC. Variety MAVERICK had got the best reaction to water stress and even more noticeable resistance to the stress was monitored in the variant with Nitrazon application in the foregoing seed treatment of soybean seeds. The opposite response to the inoculation was monitored by IMPALA genotype when RWC had dropped to 41.77% in the comparison with the variant without inoculation where RWC had dropped to 61.86%.
