Ethyl methanesulfonate (EMS), in vitro cultivation, mutagens, N-nitrose-N´-ethyl urea (ENU), Phaseolus vulgaris L.
Abstract
Infl uence of ethyl methanesulfonate (EMS) and N-nitrose-N´-ethyl urea (ENU) mutagenic treatments was investigated on three time sub-cultured calli obtained from leaf petiole explants of 7-day old sterile plants. Calibrated sterile seeds of the common bean Bulgarian variety Plovdiv 11M were pre-cultivated on MS basal medium supplemented with 1 μmM BAP. Then, both mutagens EMS and ENU were applied for different times such as 15, 30, 60 and 90 min on
the explants at the concentrations of: 2.5 . 10-2 M and 6.2 . 10-3 M, respectively. Times of the mutagenic treatments infl uenced callus growth, calli from 30-min treatment with both mutagens showing the highest weights. In both cases, the 90-min mutagen application caused a too relevant effect either on callus browning or growth inhibition. In general, ENU showed a stronger effect than EMS. The effect of subcultures on callus growth was higher than mutagenic
treatments. Interactions between these factors checked by by correlation ratio (η%) were quite low.
