The aim of this study was to determinate the actual contamination of selected area of Slovakia, in view of its environmental character referred both to the suitability or unsuitability of the use of milk from this area, to other food processing. This article deals with analysis of the content of selected compounds in soil, feed and milk, at the cow and sheep farms. Village in Eastern Slovakia, Tulčík, was the area of investigation. This area is characterized as an area with mild disturbance of environment. 11 compounds have been analyzed (calcium, selenium, cadmium, arsenic, polychlorinated biphenyls – congeners 138, 153, 180, and pesticides - p,p´ DDE, Endosulfan I., Beta-HCH, aflatoxin M1). Samples of soil were collected once a year (spring season), samples of feeds and milk were collected two-times a year (spring and autumn season). Analysis of samples was performed in Eurofins Bel/Novamann (Nové Zámky, Slovak Republic). Analyses were performed by routine methods, according to the valid methodologies. Levels of compounds were obtained and then results have been compared with the most acceptable limits in according to applicable legislation. At both farms, 73.08% (38 samples) of analyzed compounds were below the limit of quantification (LOQ) and 26.92% (14 samples) of compounds were quantifiable. The most significant differences between monitored farms were recorded in soil (27 720 mg·kg-1 Ca), feed (27 620 mg·kg-1 Ca) and milk (960 mg·kg-1 Ca). The high content of calcium in soil and feed did not affect the content of calcium in milk. The results showed that the content of toxic elements, polychorinated biphenyls, pesticides and aflatoxin M1 in analyzed area of Eastern Slovakia was very low and under the limit of quantification. It can be concluded, that the use of milk from this area for direct use or for dairy products is appropriate and poses no health risk to the consumers.