In the microbiological investigation, made on samples of normal; (n=412) and mastitic (n=482) milk,
proceeded from eight dairy cows farm, in Transilvania, it was signal the presence of the algae without
chlorophyl belonging to Prototheca, forming together with bacteria and fungi the micro flora of the mammary
gland. The alga content of normal milk was signaled only in two farms (2.27%, respectively 1.21%), being
established to 0.72%. In the case of mastitic milk the percentage of positive samples raised up to 3.3%, the
isolating frequency being important only in one farm (9.6%), where were episodes of antibiotic resistant
mastitis. The alga were well developed on gelose and Sabouraud agar, at 20-37°C, and their identification as
Prototheca, was based on making evident the typical morphological elements, big sporangium (10-30μm)
containing 4-8 daughter cells.
