Testicular toxicity induced by T-2 toxin and protective effect of vitamin E in Wistar rats

Vitamin E modulates testicular toxicity of T-2 toxin

Abstract

Background and purpose: Mycotoxicosis is a very common problem in stored feed and fodder. Ingestion of T-2 toxin through feedstuff leads to serious consequences and production losses in exposed populations. That may lead to reproductive organ system damage resulting in the reproductive problem. The study was planned to investigate the effect of T-2 toxin and vitamin E on testicular function in Wistar rats.

Material and methods: Male Wistar rats were fed with standard rat feed. T-2 toxin treatment groups received T-2 toxin (2.5 ppm and 5.0 ppm) mixed feed, while vitamin E (100 mg/kg) was given by oral gavage alone and in T-2 toxin fed groups. After completion of 28 days of oral feeding of text compounds reproductive toxicity parameters were evaluated.

Results: Total live sperm counts, sperm motility, and serum testosterone levels were reduced in T-2 toxin-treated groups. Antioxidant enzymes/nonenzymes and oxidative stress indicators were altered significantly and cellular damage to testis and epididymis in T-2 toxin groups was in a dosedependent manner. However, vitamin E intervention caused a marked reversal effect in T-2 toxin-fed rats on testicular damage and male reproductive parameters. Oxidative stress and related events appear to be a potential mechanism involved in T-2 toxin-mediated testicular damage.

Conclusion: This study validated that the use of an optimum dose of vitamin E has the potential to protect against the testicular damage caused by the T-2 toxin.