The effect of subminimal inhibitory concentrations of antibiotics on the adherence ability of Pseudomonas aeruginosa to epithelial cells in vitro

Effect of antibiotics on bacterial adherence

Authors

  • Sanja Bekić Sanja Bekić, MD, MSc, Family Medicine Practice, Osijek, Croatia; Department of Internal Medicine, Family Medicine and History of Medicine, Faculty of Medicine, University of Osijek, Osijek, Croatia
  • Domagoj Drenjančević Department of Transfusion Medicine, Osijek University Hospital, Osijek, Croatia; Department of Microbiology and Parasitology, Faculty of Medicine, University of Osijek, Osijek, Croatia
  • Jasminka Talapko Department of Microbiology and Parasitology, Faculty of Medicine, University of Osijek, Osijek, Croatia; Faculty of Dental Medicine and Health, University of Osijek, Osijek, Croatia
  • Ivan Kotris Department of Internal Medicine, General County Hospital Vukovar, Vukovar, Croatia
  • Ivana Škrlec Faculty of Dental Medicine and Health Osijek
  • Jasmina Vraneš Department of Microbiology and Parasitology, School of Medicine, University of Zagreb, Zagreb, Croatia; Department of Microbiology, Andrija Stampar Teaching Institute of Public Health, Zagreb, Croatia

DOI:

https://doi.org/10.18054/pb.v120i2-3.6749

Abstract

Background and purpose: The aim of this study was to examine the influence of subminimal inhibitory concentrations (subMICs) of ceftazidime, ciprofloxacin and gentamicin on the adherence ability and morphology of wild-type Pseudomonas aeruginosa strains to the Buffalo green monkey kidney cell line, using indirect immunofluorescence staining.

Materials and methods: Bacterial adherence changes were tested before and after exposure to 1/2, 1/4, 1/8, 1/16 and 1/32 MIC of antibiotics.

Results: A statistical difference in the number of attached bacteria after exposure to all subMICs of ceftazidime and ciprofloxacin was observed (p<0.05), even after only 1/2 MIC of gentamicin.

Conclusion: The results of this study have shown that antibiotics in much lower concentrations than those necessary for inhibition of bacterial multiplications could damage the adherence of Pseudomonas aeruginosa to the epithelial cell line.

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Published

2019-09-15

Issue

Vol. 120 No. 2-3 (2018)

Section

Articles

License

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