Nondestructive detection and biochemical quantification of buckwheat leaves using visible (VIS) and near-infrared (NIR) hyperspectral reflectance imaging
Authors
Oksana SYTAR
Department of Plant Physiology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic
2SRL "Physiological bases of plant productivity", Educational and Scientific Center “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, Volodymyrska 64, 01601, Kyiv, Ukraine
Klaudia BRÜCKOVÁ
Department of Plant Physiology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic
Marek KOVÁR
Department of Plant Physiology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic
Marek ŽIVČÁK
Department of Plant Physiology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic
Irene HEMMERICH
Department of Food Biotechnology and Food Process Engineering, Institute of Food Technology and Food Chemistry TU Berlin, Königin-Luise Str. 22, 14195 Berlin, Germany
Marián BRESTIČ
Department of Plant Physiology, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic
The present experimental study has been investigated way of use methodology of nondestructive detection with hyperspectral reflectance imaging together with wet chemistry quantitative analysis based on correlation analysis of receiving data. Higher correlation from reflectance of hyperspectral imaging analysis and total polyphenol and anthocyanin content has been observed for carotenoid reflectance index-2 compared to the carotenoid reflectance index-1. It was found that high total polyphenols content is related with high values of hyperspectral indices which characterize chlorophyll concentration and parameters of vegetation. In experimental buckwheat cultivars presence of vanillic, methoxycinnamic, cinnamic acids can be expressing by anthocyanin reflectance index, modified anthocyanin reflectance index and carotenoid reflectance index-2 because high correlation coefficient. Changes of chlorogenic acid during the vegetative period of plant growth can be studied with normalized difference vegetation index and normalized difference vegetation index-2 which found to have a positive correlation with this phenolic acid content.
