Medicina Fluminensis, Vol. 47 No. 3, 2011.
Pregledni rad
Electron microscopy in diagnostic practice
Sanja Štifter
orcid.org/0000-0001-8678-5483
; Zavod za patologiju, Medicinski fakultet Sveučilišta u Rijeci, Rijeka
Gordana Đorđević
; Zavod za patologiju, Medicinski fakultet Sveučilišta u Rijeci, Rijeka
Andrea Dekanić
orcid.org/0000-0003-0625-7483
; Zavod za patologiju, Medicinski fakultet Sveučilišta u Rijeci, Rijeka
Đuro Vranešić
; Zavod za patologiju, Medicinski fakultet Sveučilišta u Rijeci, Rijeka
Nives Jonjić
orcid.org/0000-0003-2995-0766
; Zavod za patologiju, Medicinski fakultet Sveučilišta u Rijeci, Rijeka
Sažetak
The aim of the present review is to highlight the importance, placement and availability
of the electron microscopy (EM) analyses in routine diagnostic work with biopsy samples.
Electron microscopy is a relatively new method dating from the 1970es, when it was primarily used in the diagnosis of tumors of unknown primary sites. Today it has a much wider application, such as in kidney, congenital, metabolic and accumulation diseases, neuromuscular
disorders and numerous other conditions. Prerequisite for adequate EM analysis
is good knowledge of ultrastructural cellular formation of which the article gives a brief review. In addition, the pre-analytical phase for EM analysis, which includes proper sampling,
adequate processing of tissue and acceptable the sections, necessary for satisfactory analysis is also described in the paper. As the method is the method is relatively complex and financially demanding the use of EM should be. Finally, the article describes some of the most important indications for the use EM in routine work, documented with images, such as in glomerular and muscular diseases and tumor pathology, where light microscopy and immunohistochemical analysis, are very often not sufficient for the final diagnosis.
Ključne riječi
diagnostics; electron microscopy
Hrčak ID:
71752
URI
Datum izdavanja:
5.9.2011.
Posjeta: 3.932 *