Original scientific paper

Serological, cytopathological and cytochemical studies on hydropericardium syndrome virus.

Parimal Roy ; Centre for Animal Health Studies Tamil Nadu, Veterinary and Animal Sciences University, Madhavaram Milk Colony, India
Agatheeswaran Koteeswaran ; Centre for Animal Health Studies Tamil Nadu, Veterinary and Animal Sciences University, Madhavaram Milk Colony, India
Ramagounder Manickam ; Centre for Animal Health Studies Tamil Nadu, Veterinary and Animal Sciences University, Madhavaram Milk Colony, India

Abstract

Hydropericardium syndrome (HPS) is an emerging disease of broiler chickens. For diagnosis of the disease agar gel immunodiffusion (AGID) and counter immuno-electrophoresis (CIE) were found useful. Fifty percent liver homogenate from affected chickens showed AGID titre of 3.2±0.2 log2 and CIE titre of 4.0±0.0 log2 with HPS antiserum. The causative virus became adapted in VERO cell line after four blind passages and after 96 hours produced appreciable cytopathogenic effects (CPE) characteristic of an adenovirus. Immunoperoxidase test (IPT) was carried out on coverslip cultures. Infected coverslips treated with HPS antiserum showed IPT positive reaction but the reaction was negative when coverslips were treated with Newcastle disease virus antiserum or Infectious bursal virus antiserum. Aetiology of HPS is discussed.

Keywords

hydropericardium; broilers; immuno peroxidase test; agar gel immunodiffusion; counter immuno electrophoresis

Hrčak ID:

84690

URI

https://hrcak.srce.hr/84690

Publication date:

21.4.2001.

Article data in other languages: croatian

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